Effects of Trace Metal Ions on the Growth, Homoacetogenesis and Corrinoid Production by Clostridium thermoaceticum
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概要
- 論文の詳細を見る
The influence of metal ions such as Fe^<2+>, Se^<4+>, Mo^<6+>, and Co^<2+> on the growth, homoacetogenesis and corrinoid production by Clostridium thermoaceticum was investigated in a glucose-minimal medium. Fe^<2+>, Se^<4+> and Mo^<6+> were absolutely required for growth and homoacetogenesis, giving the maximum activities of formate dehydrogenase and of carbon monoxide dehydrogenase. Growth and corrinoid formation were regulated by the presence of Fe^<2+> in the culture, and its optimum concentration was 100 μM. Although Co^<2+> did not have so much influence on the growth, homoacetogenesis and its acetate/glucose molar yield at concentrations between 0-400 μM, the maximum total corrinoid of 9.1 mg/l was produced at 400 μM of cobalt nitrate. Electrophoretic identification of the corrinoids revealed that about 22% of total corrinoids was comprised of the Factor IIIm (5-methoxybenzimidazolyl cobamide) and the remainder were mainly the base-free Factor B (cobinamide) ant its analogues.
- 公益社団法人日本生物工学会の論文
- 1991-03-25
著者
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Nishio N
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nagai Seichiro
Shikoku Milk Product Co. Ltd.
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Nagai S
Yaegaki Technology Department Laboratories Yaegaki Bio-industry Inc.
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Nagai Shiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima Univeisity
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