A Novel Method for Measurement of α-Amylase Activity in Rice Koji by Capillary Isoelectric Focusing
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概要
- 論文の詳細を見る
A capillary isoelectric focusing(CIEF) method has been developed for the analysis of α-amylase activity in rice koji. The standards of α-amylase and rice koji extracts were successfully separated by CIEF in a large pH gradient. The α-amylase from A.oryzae was found to have pI of 3.7, as determined from the migration times of six standard proteins. A simple method has been developed for the measurement of α-amylase activity in rice koji, and the results were compared with those obtained by the official method. The coefficient of variation(C.V.) of the α-amylase assay was less than 0.5% in both intra-day and inter-day experiments. This CIEF method can be applied to the routine quality control of α-amylase activity in rice koji for the brewing process in sake production.
- 社団法人日本生物工学会の論文
- 1998-04-25
著者
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寺部 茂
姫路工大・理
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寺部 茂
姫路工業大学大学院理学研究科
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TERABE Shigeru
Faculty of Science ,Himeji Institute of Technology
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寺都 茂
姫路工業大学理学部
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Nagai Shiro
Yaegaki Bio-industry Corporation
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Nagai Seichiro
Shikoku Milk Product Co. Ltd.
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Nagai S
Yaegaki Technology Department Laboratories Yaegaki Bio-industry Inc.
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WATANABE Toshiro
Yaegaki Technology Development Laboratories, Yaegaki Bio-industry, Inc.
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YAMAMOTO Akira
Yaegaki Technology Development Laboratories, Yaegaki Bio-industry, Inc.
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Yamamoto A
Meteorological Research Institute
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Watanabe Toshiro
Yaegaki Bio-industry Inc.
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TERABE Shigeru
Faculty of Science , Himeji Institute of Technology
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