Formation and Regeneration of Protoplast from a Unicellular Green Alga Haematococcus pluvialis
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概要
- 論文の詳細を見る
An effective method of preparing osmotically-labile protoplasts from Haematococcus pluvialis was established. Microalgal cells grown semi-synchronously under weak blue light was most effective in obtaining viable protoplasts with Proteinase K (0.06%) treatment in the presence of 0.2 M sorbitol/mannitol. The frequency of the osmotically-sensitive cells reached maximum in as short as 15 min protease treatment at 35℃. Protoplast formation was also verified from structural changes of the algal cells under microscopic observation. Protoplast regeneration was successfully carried out by stepwise reduction of sorbitol/mannitol concentrations from 0.2 M to 0.05 M followed by incubation on a plate under various osmotic condition. This stepwise reduction of the osmosis enabled the protoplasts to regenerate the algal cell wall with significantly improved yield.
- 社団法人日本生物工学会の論文
- 1993-03-25
著者
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NAGAI SHIRO
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Nagai Seichiro
Shikoku Milk Product Co. Ltd.
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Nagai S
Yaegaki Technology Department Laboratories Yaegaki Bio-industry Inc.
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Nagai Shiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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KAKIZONO TOSHIHIDE
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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TJAHJONO AGUS
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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HAYAMA YACHIYO
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Hayama Yachiyo
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kakizono T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kakizono Toshihide
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Tjahjono Agus
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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