SCP Production by Mixed Culture of Rhodocyclus gelatinosus and Rhodobacter sphaeroides from Cassava Waste

概要

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The amylolytic enzymes produced by Rhodocyclus gelatinosus hydrolyzed cassava starch mainly to maltose and a small amount of glucose. The organism utilized maltose at the specific growth rate of 0.15 l/h, but in the presence of glucose, maltose consumption rate was retarded. Therefore, a series of mixed cultures was conducted with Rhodobacter sphaeroides P47,which showed a high growth rate of 0.24 l/h on glucose and contained 29.5 μg/g cell of vitamin B12 and 0.49 mg/g cell of carotenoid compared with the 18.4 μg/g cell and 0.23 mg/g cell respectively of Rc. gelatinosus. Mixed cultures with three different inoculum raios of the two organisms based on cell number all gave higher growth yields and contents of vitamin B12 and carotenoid in the total cell mass than single cultures. When the inoculum ratio of Rc. gelatinosus to Rb. sphaeroides P47 was over 1.0,the culture time was shortened due to the synergistic effect of sugar consumption. THerefore, it was suggested that the mixed culture of these two organisms would be practically profitable for more nutritive SCP production from cassava waste.
社団法人日本生物工学会の論文
1987-02-25