Measurement of Cell Density in Cultures of Aggregative Organisms by Continuous-Dilution-Photometric-Assay
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概要
- 論文の詳細を見る
A cell density photometric measurement system developed previously was applied to cultures of the aggregative organisms Favolus arcularius and Streptomyces griseus. The system consisted of a photometer with a flow cell, two tubing pumps and a magnetic circulating pump. The culture broth was introduced from a culture flask to the measurement system by a tubing pump, and diluted with water fed by another tubing pump. The diluted broth was circulated by the circulating pump for several minutes to break up the cell aggregates into small pieces. The cell suspention was circulated through the flow cell and diluted with water continuously. The cell density of the culture broth was calculated based on the optical density, sampled volume of the culture broth, water flow rate for dilution, and dilution time, and then converted to dry cell mass concentration by a predetermined calibration curve. The effects of the operating conditions on calculation of the cell density were studied to select the optimum condition. The values of cell densities measured by this method gave good agreement with the values obtained by the drying method over a wide range of cell densities from 5 to 45 g dry cells/l.
- 社団法人日本生物工学会の論文
- 1993-08-25
著者
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YANO TAKUO
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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NISHIZAWA YOSHINORI
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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Nishizawa Y
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nishizawa Y
Yamaguchi Univ. Ube Jpn
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Nishizawa Yoshinori
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Nishizawa Yoshinori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Yano T
Hiroshima City Univ. Hiroshima Jpn
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Yano Takuo
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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Masduki Agus
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Yano Toshihiro
Department Of Food Science Ishikawa Agricultural College
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OKANO KENJI
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Okano Kenji
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Okano Kenji
Department Of Biotechnology Graduate School Of Engineering Osaka University
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