Fed-batch Culture of Methanol-utilizing Bacterium with DO-stat

概要

論文の詳細を見る
In a batch culture of methanol-utilizing microorganisms, the dissolved oxygen tension increased abruptly when methanol in the medium became the growth limiting factor, and then decreased to the former level on feeding methanol. These phenomena were used as the indicator on feeding methanol to a fed-batch culture. The methanol concentration was maintained within a range suitable for growth. The procedure for the control is as follows; two impeller rotation speeds were set and ON-OFF controlled in order to keep the dissolved oxygen tension constat, and the methanol feeding pump was switched on by a signal of the abrupt increase of dissoleved oxygen tension. In the batch culture, cell density was about 45g dry cell per l (initial methanol concentration was 2 % v/v), but by feeding of methanol and other nutrients using this feeding system the cell density reached 85 g dry cells per l, which corresponds to 39% of the limit that can be cultivated in view of fluid dynamics.
社団法人日本生物工学会の論文
1978-08-25