On-Line Control of Glucose Concentration Using An Automatic Glucose Analyzer

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The fundamental characteristics of an automatic glucose analyzer which consists of sampling, sensor, and operation units were examined. The glucose sensor is a dual cathode type which has an immobilized glucose oxidase membrane coupled with an oxygen sensor. Using this glucose sensor combined with an automatic sampling device, on-off control of the glucose concentration in fed-batch cultures of Saccharomyces cerevisiae was carried out. When the glucose concentration to be controlled was set as 0.3 and 10 g/l, the concentration was well maintained within the range of 0.08-0.54 g/l in the former, and within 9.2-11.1 g/l in the latter. In the former experiment, 1.67 g/l of ethanol was produced at the end of cultivation (OD_<570>=34). On the other hand, 12.9 g/l of ethanol was accumulated at the end of cultivation (OD_<570>=43) in the latter experiment. Fed-batch cultures of Micrococcus ruteus were also carried out. The glucose concentration was set at 2.5 g/l. The microorganism grew up to OD_<610>=264 and the glucose concentration was maintained within 2.0 and 3.1 g/l.
社団法人日本生物工学会の論文
1987-06-25