KEY PARAMETERS OF SPERM MOTION IN RELATION TO MALE FERTILITY IN RATS GIVEN α-CHLOROHYDRIN OR NITROBENZENE
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概要
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This study was undertaken to detect key parameters of rat sperm motion in relation to male fertility by comparing the differences in sperm motion induced by treatment with α-chlorohydrin (ACH), known to produce spermatotoxicity, and nitrobenzene (NTB), known to produce testicular toxicity. Male rats received ACH (5 or 20 mg/kg/day) or NTB (60 mg/kg/day) for either 3 days or 18 days. Epididymal sperm was assessed for motility using a Hamilton-Thorne Sperm Analyzer (HTM-IVOS). Numerical data for statistical analysis and graphical renditions of sperm motion using parameters in radar charts and reconstructed sperm tracks were analyzed to evaluate sperm motion. Males were allowed to copulate with untreated females and cesarean sections were conducted in order to examine the effects of drug administration on male fertility. Linearity of sperm track (linearity (LIN) and/or straightness (STR)) decreased and/or beat cross frequency (BCF) increased only in ACH groups (5 or 20 mg/kg/day), although the percentage of motile sperm, sperm velocities (average path velocity (VAP), curvilinear (VCL), and straight line velocity (VSL)) and amplitude of lateral head displacement (ALH) decreased on Day 18 in both ACH and NTB (60 mg/kg/day) groups. Furthermore, from the individual reconstructed sperm tracks, it was clear that ACH-treated spermatozoa were characterized by abnormal motion ("jerking") with low vigor (low velocities) and little or no forward progression. Finally, only ACH treatment led to a reduction in pregnancy rate or infertility. Therefore, our results suggest that linearity (especially VSL, STR and LIN) in sperm motion is a key parameter for assessing a chemical's potential to induce male infertility.
- 2004-08-30
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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KAWACHI Mamoru
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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MORIKAWA Makoto
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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KAZUTA Hiroki
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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SHIBATA Kenji
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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ISHIDA Mayumi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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KITAGAWA Naoko
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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MATSUO Asaki
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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KADOTA Toshihito
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Matsuo Asaki
日本ベーリンガーインゲルハイム川西医薬研究所
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Matsuo Asaki
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Ishida Mayumi
Department Of Breast Oncology National Kyushu Cancer Center
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Ishida Mayumi
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Ishida Mayumi
日本ベーリンガーインゲルハイム川西医薬研究所
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Kazuta Hiroki
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Kazuta Hiroki
日本ベーリンガーインゲルハイム川西医薬研究所
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Kitagawa Naoko
日本ベーリンガーインゲルハイム川西医薬研究所
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Kitagawa Naoko
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Kawachi Mamoru
日本ベーリンガーインゲルハイム川西医薬研究所
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Kawachi Mamoru
Dept. Of Toxicol. & Safety Assess. Kawanishi Pharma Res. Institute Nippon Boehringer Ingelheim C
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Kawachi Mamoru
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Shibata Kenji
Department Of Neurosurgery Matsue Red Cross Hospital
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Morikawa Makoto
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Morikawa Makoto
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Kadota Toshihito
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Shibata Kenji
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Toxicology And Safety Assessment Kawanishi Pharma Research Institute Nippon Boehringer
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Shibata Kenji
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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