Efficient Regeneration from GUS-Transformed Ajuga Hairy Root
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概要
- 論文の詳細を見る
A procedure for the systematic regeneration from Ajuga hairy root was developed. Supplementation of an auxn, naphthaleneacetic acid (0.1 mg/l), in the hairy root growth culture enhanced the cell growth rate. Mechanical fragmentation for 10 s in a commercial blender enabled a large number of root fragments to be produced efficiently. Supplementation of a cytokinin, benzyladenine (10 mg/l), exhibited the highest plantlet formation frequency in the plantlet formation stage. The plantlets longer than 4 mm in length were able to grow into whole plants on agar containing modified MS medium. Akuga hairy root in which the β-glucuronidase (GUS) gene was introduced undr control of the tomato ribulose-1,5-gisphosphate carboxylase/oxygenase small subunit (rbcS3B) promoter was constructed by the Agrobacterium rhizogenes-mediated co-transformation. The GUS-transformed hairy root could also be efficiently regenerated into plantlets through this procedure. GUS activity was detected in leaf tissue of the regenerated plants.
- 1996-05-25
著者
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Tanaka Nobukazu
Center for Gene Science, Hiroshima University
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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KOBAYASHI TAKESHI
(Present address) Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Uozumi N
Nagoya Univ. Nagoya Jpn
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Tanaka Nobukazu
Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Kobayashi T
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Kobayashi T
Univ. Tsukuba Ibaraki Jpn
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Kobayashi Takeshi
Dept.of Chemical Engineering Nagoya University
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UOZUMI NOBUYUKI
(Present address)Bioscience Center, Nagoya University
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OHTAKE YASUKI
Department of Biotechnology, Faculty of Engineering, Nagoya University
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NAKASHIMADA YUTAKA
(Present address)Department of Fermentatin Technology, Faculty of Engineering, Hiroshima University
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MORIKAWA YUTAKA
Department of Biotechnology, Faculty of Engineering, Nagoya University
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Morikawa Yutaka
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Nakashimada Y
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Ohtake Yasuki
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kobayashi Takeshi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Kobayashi Takeshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kobayashi Takeshi
Department Of Biotechnology:faculty Of Engineering Nagoya University
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KOBAYASHI TAKESHI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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KOBAYASHI TAKESHI
Department of Biotechnology, Faculty of Engineering, Nagoya University
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