Expression of Betaine Aldehyde Dehydrogenase Gene in Transgenic Tomato Hairy Roots Leads to the Accumulation of Glycine Betaine and Contributes to the Maintenance of the Osmotic Potential under Salt Stress
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概要
- 論文の詳細を見る
Genetically engineered tomato hairy root lines with the ability to synthesize glycine betaine were established by introducing the betaine aldehyde dehydrogenase gene (BADH-1) from sorghum plants. The lines showed a mikimopine activity, confirming the transformation with pRi plasmid. The stable integration of the BADH gene into the tomato genome was confirmed by Southern blotting analysis. The expression of BADH mRNA could be detected in the tomato hairy roots by Northern analysis. The osmotic potential (Ψ_s) of the transgenic hairy roots was sufficient to allow water uptake. The accumulation of betaine was detected by ^1H-NMR analysis. The accumulation of betaine in the transgenic hairy roots contributed to the maintenance of the osmotic potential of the cells.
- 社団法人日本土壌肥料学会の論文
著者
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Tanaka Nobukazu
Center for Gene Science, Hiroshima University
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Saneoka Hirofumi
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Science Hiroshima U
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Saneoka Hirofumi
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Sciences Hiroshima
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Saneoka Hirofumi
Graduate School Of Biosphere Sci. Hiroshima Univ. 1-4-4 Kagamiyama Higashi-hiroshima 739-8528 Jpn
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Moghaieb Reda
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Science Hiroshima U
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Fujita K
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Science Hiroshima U
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Fujita K
Graduate School Of Biosphere Science Hiroshima University
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Fujita K
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Sciences Hiroshima
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Fujita Kounosuke
Department of Environmental Dynamics and Management, Graduate School of Biosphere Sciences, Hiroshim
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Hussein Hashem
Department of Genetics and Genetic Engineering Center, Faculty of Agriculture, Cairo University
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Yousef Sawsan
Department of Genetics and Genetic Engineering Center, Faculty of Agriculture, Cairo University
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Ewada Mohamed
Department of Genetics and Genetic Engineering Center, Faculty of Agriculture, Cairo University
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Aly Mohamed
Department of Genetics and Genetic Engineering Center, Faculty of Agriculture, Cairo University
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Tanaka Nobukazu
Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn
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Tanaka N
Center For Gene Science Hiroshima University
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Fujita Kounosuke
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Science Hiroshima U
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Aly Mohamed
Department Of Genetics And Genetic Engineering Center Faculty Of Agriculture Cairo University
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Yousef Sawsan
Department Of Genetics And Genetic Engineering Center Faculty Of Agriculture Cairo University
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Ewada Mohamed
Department Of Genetics And Genetic Engineering Center Faculty Of Agriculture Cairo University
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Hussein Hashem
Department Of Genetics And Genetic Engineering Center Faculty Of Agriculture Cairo University
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