Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1

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概要

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• Tobacco BY-2 cells were transformed with the human UDP-galactose transporter 1 gene (hUGT1) under the control of a 35S promoter. Accumulation of mRNA and protein derived from hUGT1 was detected in the hUGT1-transformed BY-2 cells. To identify the cell surface carbohydrates, BY-2 protoplasts were treated with 21 kinds of biotinylated lectin, stained with fluorescein isothiocyanate (FITC)-conjugated streptavidin and observed by fluorescence microscopy. FITC-ConA and -RCA120 staining exhibited strong fluorescence on the surface of BY-2 protoplasts, whereas staining with other FITC-lectin conjugates showed faint or no fluorescence. Regarding FITC-ConA and -RCA120 staining, the fluorescence of hUGT-expressing BY-2 protoplasts was weaker than that of control cells. Decreased FITC fluorescence of hUGT-expressing BY-2 protoplasts was also detected by fluorescence-activated cell sorting (FACS) analysis, suggesting that the surface carbohydrate composition was modified.

• 2009-09-25

著者

• Ishida Nobuhiro

  Department of Environmental Security Systems, Faculty of Risk and Crisis Management, Chiba Institute

• Tanaka Nobukazu

  Center for Gene Science, Hiroshima University

• Khalil Mohamed

  Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn

• Tanaka Nobukazu

  Center For Gene Sci. Hiroshima Univ. 1-4-2 Kagamiyama Higashi-hiroshima Hiroshima 739-8527 Jpn

• Kawahara Tetsuya

  Center for Gene Science, Hiroshima University

• HORIBE Takahiro

  Center for Gene Science, Hiroshima University

• Kawahara Tetsuya

  Center For Gene Science Hiroshima University

• Ishida Nobuhiro

  Dep. Of Environmental Security Systems Fac. Of Risk And Crisis Management Chiba Inst. Of Sci. 3 Shio

• Ishida Nobuhiro

  Department Of Biochemical Cell Research

• Horibe Takahiro

  Center For Gene Science Hiroshima University

関連論文

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