Hairy Root-mediated Transgenic-Plant Regeneration in Egyptian Clover (Trifolium alexandrinum L.)

概要

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Transformed plants of Egyptian clover (berseem clover, Torifolium alexandrinum L.) were obtained from hairy roots induced by infection with Agrobacterium rhizogenes strain DC-AR2 (harboring mikimopine-type pRi1724). Among established 16 hairy root lines showing growth variations on MS medium containing 0.5 mg l-1 NAA, two lines with moderate or slow proliferation regenerating shoots spontaneously. The presence of T-DNA of pRi1724 in the genome of the regenerated shoots was confirmed by the DNA gel blot analysis. The regenerated shoots repeatedly formed shoots but scarcely formed roots on hormone-free MS. Whole plants were obtained from the shoots when they were cultured on Florialite for two months. The regenerated plants displayed the hairy root syndrome such as dwarfing and alterations of leaf shape. We propose here that hairy root-mediated regeneration is one of the effective tools for obtaining transgenic plants of Egyptian clover.
日本植物細胞分子生物学会の論文
2004-06-01