Secretion of Thermophilic Bacterial Cellobiohydrolase in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
The partial DNA sequence corresponding to the N-terminal amino acid sequence of cellobiohydrolase derived from a thermophilic anaerobe NA10 was determined. The cellobiohydrolase gene fused to the secretion signal (signal peptide and T-S region) from Saccharomyces diastaticus was expressed in an ethanologenic yeast, S. cerevisiae YIY345,under control of the glucoamylase promoter. The recombinant yeast produced cellobiohydrolase : approximately 40% of the total cellobiohydrolase activity was detected in the medium, and the remaining cellobiohydrolase was localized in the intracellular fraction. An analysis of the N-terminal amino acid sequence of the main intracellular cellobiohydrolase revealed that the signal peptide and T-S region were removed proteolytically. Alteration of the amino acid residues at the cleavage site by insertion of a Bgl II linker led to an approximately 3.5-fold increase in the total cellobiohydrolase production, but did not affect the efficiency of secretion into the medium. Cellobiohydrolase production was not repressed in the presence of glucose. The recombinant yeast hydrolyzed carboxymethyl cellulose in the medium. The results suggest the possibility of the direct bioconversion of cellulose to ethanol by the recombinant yeast.
- 社団法人日本生物工学会の論文
- 1993-06-25
著者
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YAMASHITA Ichiro
Center for Gene Science, Hiroshima University
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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HAYASHI AKIHIRO
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Uozumi N
Nagoya Univ. Nagoya Jpn
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Yamashita Ichiro
Center For Gene Science Hiroshima University
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Hayashi Akihiro
Department Of Biotechnology Faculty Of Engineering Nagoya University
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UOZUMI NOBUYUKI
Department of Biotechnology, Faculty of Engineering, Nagoya University
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ITO TAKAOMI
Department of Biotechnology, Faculty of Engineering, Nagoya University
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PATTHRA ARUNWANICH
Department of Biotechnology, Faculty of Engineering, Nagoya University
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Ito T
Food Technol. Res. Inst. Nagano Prefecture Nagano Jpn
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Kobayashi T
Univ. Tsukuba Ibaraki Jpn
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Uozumi Nobuyuki
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Patthra Arunwanich
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Arunwanich Patthra
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Yamashita Ichiro
Center Of Gene Science Hiroshima University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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