Purification of Recombinant α-Amylase with Immuno-Affinity Chromatography Using Monoclonal Antibody

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概要

• 論文の詳細を見る

• A monoclonal antibody against recombinant thermostable α-amylase produced by Escherichia coli was isolated from serum-free medium and immobilized on Sepharose 4B. The adsorption equilibrium between α-amylase and the immobilized immuno-adsorbent showed a Langmuir type isotherm. The breakthrough curve calculated numerically using the averaged volumetric coefficient coincided well with the experimental date. More than 90% of the activity of bound α-amylase could be recovered by eluting with glycine-HCl buffer (pH 2.5). The elution profile at pH 2.5 became sharper with increasing temperature. By using an immuno-affinity column, the recombinant α-amylase produced by E. coli could be purified homogeneously from crude extract enzyme solution with two-step elution.

• 社団法人日本生物工学会の論文
• 1988-12-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Taniguchi M

  Fac. Engineering Niigata Jpn

• Taniguchi M

  Department Of Material Science And Technology Faculty Of Engineering

• Taiyouji Mayumi

  Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura

• Taniguchi Masayuki

  Department Of Materials Science And Technology Niigata University

• TANIGUCHI MASAYUKI

  (Present address)Department of Chemical Engineering, Niigata University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Tanaka Mitsuo

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Taniguchi Masayuki

  (present Address)department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Takahashi Masafumi

  Department of Materials Science and Technology, Niigata University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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