Kinetic Study of Hybridoma Metabolism and Antibody Production in Continuous Culture Using Serum-Free Medium
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概要
- 論文の詳細を見る
Effects of glucose and glutamine on metabolism and antibody production were investigated in continuous culture of hydridoma cells using a serum-free medium. Viable cell concentration remained virtually constant during culture. The production of inhibitory metabolites, such as lactate and ammonium, could be reduced by keeping the concentration of glucose or glutamine in the medium at a low level. The specific production rate of antibody at a low glucose or glutamine concentration was 3-fold higher than that at high concentrations of glucose and glutamine (greate than 2.5 mM and 1.0 mM, respectively). The results obtained in this study suggest that maintaining the concentrations of glucose and glutamine at low levels in a hydridoma culture is important for reducing the formation of inhibitory metabolited and to maintain high antibody productivity.
- 社団法人日本生物工学会の論文
- 1993-08-25
著者
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Park Yong
Department of Genetic Engineering, Kyungpook National University
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OGAWA Tatsuya
Department of Cardiovascular Surgery, Kinki University School of Medicine
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Park Y
Shizuoka Univ. Shizuoka Jpn
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Park Yong
(present Address)department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Universi
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Park Yong
Biochemical Eng. Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Uni
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Park Yong
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kirinaka Hideyo
Department of Biotechnology, School of Engineering, Nagoya University
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KUROKAWA HIDEO
Department of Biotechnology, Faculty of Engineering, Nagoya Univeersity
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Kurokawa Hideo
Department Of Biotechnology Faculty Of Engineering Nagoya Univeersity
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Kurokawa Hideo
Department Of Applied Physics Faculty Of Technology Tokyo University Of Agriculture And Technology
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Ogawa T
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kobayashi T
Univ. Tsukuba Ibaraki Jpn
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Ogawa Tatsuya
Department Of Cardiovascular Surgery Kinki University School Of Medicine
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Kirinaka Hideyo
Department Of Biotechnology School Of Engineering Nagoya University
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Ogawa Tatsuya
Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya University
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Park Yong
Department of Advanced Materials, Engineering for Information and Electronics, Kyung Hee University, Yongin 446-701, Korea
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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Park Yong
Department of Advanced Materials Engineering, Kyonggi University, Suwon, Gyeonggi-do 443-760, Republic of Korea
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