Inducible Production of Erythropoietin Using New Runaway-Type Vector by Mammalian Cell Culture in Spinner Flask

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概要

• 論文の詳細を見る

• Human erythropoietin (HuEPO) gene was inserted into an SV40-based episomal vector with a temperature-sensitive large T antigen which enabled nucontrolled plasmid replication and inducible HuEPO production at temperatures below 33℃. Good productibity of HuEPO was obtained when temperature was lowered to 30℃ at relatively higher cell density in Petri dish culture. Therefore, we cultured the cells immobilized on microcarriers in a spinner flask at 37℃ unitl the cell density reached 1.8×10^4 cells/cm^2-microcarrier surface area, and then the temperature was lowered to 30℃ for HuEPO production. Using this process, HuEPO productivity was enhanced 7-18 times that of the Petri dish cultures, and a high concentration of HuEPO was accumulated in the medium.

• 社団法人日本生物工学会の論文
• 1994-12-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kirinaka Hideyo

  Department of Biotechnology, School of Engineering, Nagoya University

• KIRINAKA HIDEYO

  Depertment of Biotechnology, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kurokawa Hideo

  Department Of Biotechnology Faculty Of Engineering Nagoya Univeersity

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Kirinaka Hideyo

  Department Of Biotechnology School Of Engineering Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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