Production of Anti-Prion scFv-Fc Fusion Proteins by Recombinant Animal Cells

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概要

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• We constructed a replication-defective retroviral vector plasmid for the expression of a single-chain antibody fragment (scFv), derived from a chicken anti-human prion protein monoclonal antibody, fused with the Fc region of human IgG1. CHO-K1 and NS-I cells were transformed with the viral vector pseudotyped with vesicular stomatitis virus G protein (VSV-G), and scFv-Fc producer clones were established. Among the established clones, CHO-2A9 cells produced a large amount of the product with an antibody-like dimerized structure in serum-free culture that facilitated the purification of scFv-Fc. The scFv-Fc specifically recognized the epitope sequence of prion protein in solid-phase enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. The injection test into quails revealed that the scFv became more stable in vivo by fusion with the Fc region. The scFv-Fc will be a useful tool for the detection of mammalian prion proteins.

• 社団法人日本生物工学会の論文
• 2003-03-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kawabe Yoshinori

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawabe Yoshinori

  九州大学 工学研究院化学工学

• Kawabe Yoshinori

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Matsumoto Hiroyuki

  Tokyo Institute Of Technology

• NAKAMURA Naoto

  Department of Information Processing, Tokyo Institute of Technology

• MATSUDA Haruo

  Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima Univ

• Matsumoto Hiroyuki

  Department of Electrical Engineering, Toyo University

• Nakamura Naoto

  Department Of Endocrinology And Metabolism Kyoto Prefectural University Of Medicine Graduate School

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima S

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima S

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima Shinji

  Department Of Chemical Engineering Nagoya University

• Nishijima Ken-ichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Ono Ken-ichiro

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Nagoya University

• Hotta Akitsu

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• KUGA YUKO

  Department of biotechnology, Graduate School of Engineering, Nagoya University

• ITOH TOSHINARI

  Department of biotechnology, Graduate School of Engineering, Nagoya University

• Kuga Yuko

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Hotta Akitsu

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Matsuda Haruo

  Department Of Immunobiology Faculty Of Applied Biological Science Hiroshima University

• Itoh T

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Matsumoto H

  Tokyo Institute Of Technology Department Of Mechanical Engineering And Science

• Yoshida Kazuhiro

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl

• Nakamura Naoto

  Department Of Immunobiology Faculty Of Applied Biological Science Hiroshima University

• Matsuda Haruo

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Matsumoto Hiroyuki

  Department Of Biochemistry And Molecular Biology University Of Oklahoma Health Sciences Center

• Itoh Takashi

  Japan Collection Of Microorganisms Riken(the Institute Of Physical And Chemical Research)

• Itoh Toshinari

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Matsumoto Hiroyuki

  Department Of Biochemistry And Molecular Biology The University Of Oklahoma Health Sciences Center

• Nishijima Kenichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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