Continuous production of anti-erythropoietin antibody by immobilized hybridoma cells.
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概要
- 論文の詳細を見る
A hybridoma cell line epo2C which produces anti-erythropoietin monoclonal antibody was immobilized with alginate and urethane polymer under several conditions. Concentration of the alginate affected neither the cell growth nor the antibody production when the alginate was hardened by calcium. However, the concentration was very important when strontium was used for the hardening. By coating alginate gel with urethane polymer, a long term cultivation of the immobilized cells and stable antibody production by a fluidized bed reactor were possible without destruction of the gel particles. During the cultures, the cells leaked from the alginate gel but were trapped inside of urethane coat with any types of gel. Therefore, high cell density of more than 10<SUP>7</SUP> cells/cm<SUP>3</SUP>-gel was attained and volumetric productivity of the antibody was 0.8 mg/(cm<SUP>3</SUP>·d). This method will provide a promising way for effective and simple antibody production.
- The Society of Chemical Engineers, Japanの論文
著者
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Yoshida Hitoshi
Department Of Anesthesiology Hirosaki University Graduate School Of Medicine
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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