Characterization of Transient Expression System for Retroviral Vector Production(MEDICAL BIOTECHNOLOGY)

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概要

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• The production of retroviral vectors using a transient expression system has been improved to obtain a high-titer virus preparation that is difficult to produce using packaging cell lines due to the cytotoxic or cytostatic effect of transgenes. Here, we used one such production method, the so-called Q-vector system, and examined its potential for virus production. The Q-vector system could produce a similar level of viral vectors compared with the packaging cell system but the production seemed to depend on the size and nature of transgenes. In the process of investigation of the quantitative difference in viral components between the transient expression system and the packaging cell system, we found that the Q-vector system could express higher amounts of viral RNA and proteins compared with the packaging cell system. However, this did not lead to a higher virus titer compared with that produced by the packaging cell system. This suggests that retroviral RNA transcribed from the plasmid in the transient system seemed to be used mainly for translation and only some of the RNA molecules were packaged in viral particles.

• 社団法人日本生物工学会の論文
• 2006-04-25

著者

• Kawabe Yoshinori

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kawabe Yoshinori

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawabe Yoshinori

  Nagoya University:(present Office)kyushu University

• Kawabe Yoshinori

  九州大学 工学研究院化学工学

• Kawabe Yoshinori

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima S

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima S

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima Shinji

  Department Of Chemical Engineering Nagoya University

• Nishijima Ken-ichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Ken-ichi

  Nagoya University

• Kyogoku Kenji

  Kaneka Corporation

• Hotta Akitsu

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Saito Yoshikazu

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Saito Yoshikazu

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kuga Yuko

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Hotta Akitsu

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Yoshida Kazuhiro

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl

• Kawabe Yoshinori

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Kenichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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