Identification of Sialyltransferases of Streptococcus agalactiae

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概要

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• Group B streptococci, Streptococcus agalacliae, produce high-molecular-weight polysaccharides containing N-acetylneuraminic acid. Although the type-specific capsular polysaccharide (CP) synthesis (cps) genes of several S. agalactiae strains have been extensively analyzed, to date, no sialyltransferase activity has been detected from any gene product of the cps gene cluster. Among the eps genes, the cpsK gene products of S. agalactiae types Ia and Ib showed weak similarity to several bacterial sialyltransferases. In this study, the cpslaK and cpslbK gene products were found to show sialyltransferase activity specific for lacto-N-neotetraose and lacto-N-tetraose, respectively. This acceptor specificity seems to reflect the respective CP structure, since the repeating unit of type Ia CP is sialyllacto-N-neotetraose and that of type Ib CP is sialyllacto-N-tetraose. We also found that the C-terminal regions of CpsKs were almost completely conserved in various S. agalactiae strains.

• 社団法人日本生物工学会の論文
• 2002-06-20

著者

• 三宅 克英

  名大院・工・生物機能

• YAMAMOTO Shin

  Department of Thermonuclear Fusion Research, Japan Atomic Energy Research Institute

• WATANABE Masaki

  Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Endo Takahiko

  Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University

• Watanabe M

  Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima S

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Koizumi Satoshi

  Sensor Materials Center National Institute For Materials Science

• Koizumi Satoshi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Miyake Katsuhide

  Department of Biotechnology, School of Engineering, Nagoya University

• Sugimoto Yukihiro

  Arid Land Research Center, Tottori Univ.

• Yamamoto Shinjiro

  Department Of Bioengineering Tokyo Institute Of Technology:(present Address)department Of Applied Li

• Yamamoto S

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Watanabe M

  Graduate School Of Engineering Hiroshima Kokusai Gakuin University

• Koike Y

  Nagoya Univ. Nagoya Jpn

• Ozaki A

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Ozaki Akio

  Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Ozaki Akio

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.:(present Office)technical Research Laboratori

• KATAOKA YOHEI

  Department of Biotechnology Graduate School of Engineering, Nagoya University

• ENDO TETSUO

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd.

• Koike Yoichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Koizumi S

  Sensor Materials Center National Institute For Materials Science

• Yamamoto S

  Department Of Bioengineering Tokyo Institute Of Technology

• Miyake K

  Nagoya University

• Miyake Katsuhide

  Department Of Biotechnology School Of Engineering Nagoya University

• Koizumi Satoshi

  Advanced Materials Laboratory National Institute For Materials Science

• Watanabe Masaki

  Department Of Applied Chemistry Graduate School Of Science And Engineering Ehime University

• Yamamoto Shin

  Department Of Cardiothoracic Surgery Juntendo University School Of Medicine

• Miyake Katsuhide

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOIZUMI Satoshi

  Advanced Science Research Center, Japan Atomic Energy Research Institute

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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