Cloning and Sequence Analysis of cDNA Encoding Endopolygalacturonase I from Stereum purpureum
スポンサーリンク
概要
- 論文の詳細を見る
Endopolygalacturonase (endoPG) I was obtained from Stereum purpureum by an improved easier purification procedure. It was found that EndoPG I consisted of three glycosilated proteins with the same isoelectric point and different molecular masses, 42, 45, and 48kDa, respectively. However, the enzymatic deglycosilation product of endoPG I gave a single band at the position corresponding to 39kDa on SDS-PAGE. Furthermore, the N-terminal amino acid sequences of three endoPGs were identical one another up to 20 residues. A cDNA library was constructed and positive cDNA clones encoding endoPG I were isolated by using antibody raised against the purified endoPG I. Nucleotide sequence analysis of the cDNA disclosed a 1212-bp open reading frame that encoded 403 amino acid residues. The N-terminal amino acid sequence (residues 1-20) of endoPG I coincided with the deduced amino acid sequence starting from the 25th residue. Therefore, the sequence of the first 24 residues represented a signal peptide and the remaining sequence, consisting of 379 residues, was the mature protein with molecular mass of 39.1 kDa.The deduced sequence of endoPG I showed 30-45% similarity in comparison with those of bacterial and fungal endoPGs, and the sequence of putative active site residues reported for the endoPGs was highly conserved in the sequence of endoPG I.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
-
MIYAIRI Kazuo
Faculty of Agriculture and Life Science, Hirosaki University
-
Miyairi K
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
-
MIYAIRI Kazuo
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
-
OKUNO Toshikatsu
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
-
WATANABE Masaki
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Miyairi Kazuo
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
-
HASUI Yuji
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
-
SENDA Mineo
Hirosaki University Gene Research Center
-
Hasui Y
Osaka Municipal Technical Res. Inst. Osaka Jpn
-
Senda M
Gene Research Center Hirosaki University
-
Okuno T
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
-
Hasui Yuji
Department Of Biochemistry Osaka Municipal Technical Research Institute
-
Watanabe Masaki
Department Of Applied Chemistry Graduate School Of Science And Engineering Ehime University
-
Watanabe Masaki
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
-
Okuno Toshikatsu
Department of Agricultural Chemistry, Faculty of Agriculture, Hirosaki University
関連論文
- The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region
- Enantioselective Synthesis of Four Isomers of 3-Hydroxy-4-Methyltetradecanoic Acid, the Constituent of Antifungal Cyclodepsipeptides W493 A and B
- Antifungal Cyclodepsipeptides, W493 A and B, from Fusarium sp. : Isolation and Structural Determination
- Anti-angiogenic Effects of Dimethyl Sulfoxide on Endothelial Cells(Pharmacognosy)
- Azelnidipine, a long-acting calcium channel blocker, could control hypertension without decreasing cerebral blood flow in post-ischemic stroke patients. A ^I-IMP SPECT follow-up study
- PA92 NOVEL PHYTOTOXINS FROM THE PATHOGENIC FUNGI ON APPLE
- Purification and Characterization of an Endopolygalacturonase from Physalospora piricola
- P-42 SYNTHETIC STUDIES OF OLIGOGALACTURONATE ANALOGUES AS MOLECULAR PROBES FOR endo-PG1
- β-Selective Glycosylation of 5-Thioglucopyranose Derivatives; Syntheses of β-(1→6) Linked 5'-Thioglucopyranosyl Disaccharides
- Induction of Heme Oxygenase-1 by Aluminum and Lack of Effect of Antioxidants(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
- Differential Display of Mice Brain mRNAs Regulated by Aluminum Treatment (Proceedings of the 23rd Symposium on Toxicology and Environmental Health)
- Characterization of an endopolygalacturonase Gene cppg1 from Phytopathogenic Fungus Chondrostereum purpureum
- Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum
- Cloning and Sequence Analysis of cDNA Encoding Endopolygalacturonase I from Stereum purpureum
- Novel Exopolygalacturonases Produced by Alternaria mali
- Quinochalcones and Flavonoids from Fresh Florets in Different Cultivars of Carthamus tinctorius L.
- White Thread-Like Retinal Arterioles Associated With Antiphospholipid Antibody Syndrome
- Discrimination between Virulent and Attenuated Isolates of Tomato mosaic virus by Restriction Fragment Length Polymorphism
- Structure of Precarthamin, a Biosynthetic Precursor of Carthamin
- Cloning of a Gene Encoding Hydroxyquinol 1,2-Dioxygenase that Catalyzes Both Intradiol and Extradiol Ring Cleavage of Catechol
- Identification of Sialyltransferases of Streptococcus agalactiae
- Molecular Characterization of a Novel β1, 3-Galactosyltransferase for Capsular Polysaccharide Synthesis by Streptococcus agalactiae Type Ib^1
- Putative Regulatory Gene for Polysaccharide Synthesis of Streptococcus agalactiae Type Ia
- Screening of Bacteriophages Producing Endo-N-Acetylneuraminidase
- Novel Bis-fused π-Electron Donor Composed of Tetrathiafulvalene and Tetraselenafulvalene
- Characteristics of Progeny Plants Originating from Asymmetric Somatic Cell Hybridization of Birdsfoot Trefoil (Lotus cornicuratus L.) and Rice (Oryza sativa L.)
- Influence of PTGS on Chalcone Synthase Gene Family in Yellow Soybean Seed Coat
- Sequence divergence at chalcone synthase gene in pigmented seed coat soybean mutants of the Inhibitor locus
- Molecular Cloning and Characterization of a Subfamily of UV-B Responsive MYB genes from Soybean
- Molecular Mechanism of Seed Coat Discoloration Induced by Low Temperature in Yellow Soybean
- Fournier gangrene associated with hyper IgE syndrome (Job syndrome)
- Endogenous short interfering RNAs of chalcone synthase genes associated with inhibition of seed coat pigmentation in soybean
- Synthesis of _D-Trigalacturonic Acid Methylglycoside and Conformational Comparison with Its Sulfur Analogue
- Treatment of Keratomycosis
- Effects of Telmisartan on Insulin Resistance in Japanese Type 2 Diabetic Patients
- The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region
- GM-04 A STUDY ON FORGING OF SPIRAL BEVEL GEAR OF ARBITRARY AXIAL ANGLE(MANUFACTURING OF GEARS)
- Endovascular Interventions for Patients With Serious Symptoms Caused by Embolic Carotid T Occlusion
- Purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17
- Hyperbaric Oxygen Combined With Intravenous Edaravone for Treatment of Acute Embolic Stroke : a Pilot Clinical Trial
- Cloning of the Gene Encoding an Endo-Acting Pectate Lyase from Streptomyces thermocarboxydus
- Acute Posterior Multifocal Placoid Pigment Epitheliopathy Accompanied by Cystoid Macular Edema
- Coil Embolization of an Aneurysm Located at the Trunk of the Persistent Primitive Trigeminal Artery : Case Report
- Copper is Required for Retinoic Acid Receptor-Dependent Transcription and Neuronal Differentiation in P19 Embryonal Carcinoma Cells
- IN VITRO TESTS OF 1,3-DITHIA-2-THIOXO-CYCLOPENT-4-ENE TO EVALUATE THE MECHANISMS OF ITS HEPATOPROTECTIVE ACTION
- Pretreatment with Aluminum and Gallium Relieved the Acute Lethal Toxicity of Carbon Tetrachloride in Mice (Proceedings of the 21st Symposium on Toxicology and Environmental Health)
- Copper Binding of Nuclear RNA-and DNA-Binding Proteins p54^/NonO and PSF
- GDS-5 A STUDY ON WN GEAR WITH CIRCULAR ARC TOOTH NORMAL PROFILE(GEAR DESIGN AND SYNTHESIS)
- Suppressive mechanism of seed coat pigmentation in yellow soybean
- Glycosylation Status and Conformational Stability of Recombinant Stereum purpureum Endopolygalacturonase IVs Produced in an Aspergillus oryzae Expression System
- Active Suppression of EndoPG IV by Ligation of the Pro-Sequence from Stereum purpureum Pro-EndoPG I
- Suppressive mechanism of seed coat pigmentation in yellow soybean
- Structures and Phytotoxicity of Metabolites from Valsa ceratosperma
- The absolute configuration of 4,6-decadiyne-1,3,8-triol isolated from Gymnopilus spectabilis.
- Effect of intrathecal administration of E-series prostaglandin 1 receptor antagonist in a cyclophosphamide-induced cystitis rat model