Discrimination between Virulent and Attenuated Isolates of Tomato mosaic virus by Restriction Fragment Length Polymorphism
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概要
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The three missense mutations on the gene for the 130-K protein of Tomato mosaic virus (ToMV) L_<11> A have been thought to be responsible for the attenuation of its virulence. The Eco471 RFLP detecting the missense mutation at 2349 successfully discriminated L_<11>A and its derivative attenuated isolates from ToMV virulent ones. RFLP analysis and mismatch amplification assay detecting the missense mutations at 1117 and 2754, respectively, could not discriminate some of the attenuated isolates from the virulent ones. These results indicated that, of the three missense mutations, only the one at 2349 was conserved in all the L_<11>A-derivative attenuated isolates.
- 2001-11-25
著者
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SENDA Mineo
Hirosaki University Gene Research Center
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Yamamoto H
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Inoue Masayasu
Akita Prefectural University
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Ueda K
Ntt Atsugi‐shi Jpn
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Senda Mineo
Hirosaki University Hirosaki
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Yamamoto Hideki
Akita Prefectural Agriculture Forestry And Fisheries Res. Center Akita Jpn
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UEDA Kenji
Akita Prefectural University
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MATSUMOTO Tsutomu
Akita Prefectural College of Agriculture
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KIGUCHl Tadahiko
Hokkaido Central Agricultural Experiment Station
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MATSUMOT0 Tsutomu
Akita Prefectural College of Agriculture
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Matsumoto T
Akita Prefectural College Of Agriculture
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