Characterization and Functional Analysis of Class I and II Acidic Chitinase cDNA from Rough Lemon
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概要
- 論文の詳細を見る
Two cDNAs encoding acidic chitinase (RlemAchiI and RlemA chiII) were identified from rough lemon (Citrus jambhiri Lush.). The deduced amino acid sequence of RlemAchiI has a typical N-terminal cysteine-rich domain, a proline/glycine-rich hinge domain and a main structure, indicating that RlemAchiI encodes a class I chitinase. RlemAchiII encodes a class II acidic chitinase. RlemAchiI and RlemAchiII were expressed in Escherichia coli cells, and the fusion proteins had chitinase activity. Northern blot analysis revealed that the corresponding mRNA is inducible by wounding or microbe attack. The transcript was not constitutive but accumulated in leaves within 2 hr after wounding or inoculation with nonpathogenic isolates of Alternaria alternata. The RlemAchi was also induced, but appearance of the signal was delayed after inoculation with the rough lemon pathotype of A. alternata, which is pathogenic to rough lemon and produces a host-selective ACR-toxin.
- 日本植物病理学会の論文
- 2002-08-25
著者
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Yamamoto Hiroyuki
Laboratory of Bioorganic Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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Akimitsu K
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Akimitsu K
Tottori Univ. Tottori Jpn
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Akimitsu Kazuya
Laboratories Of Plant Pathology
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Akimitsu Kazuya
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Yamamoto H
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Gomi Kenji
United Graduate School Of Agricultural Sciences Kagawa University
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ITOH Namiko
Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture ,Kagawa Universit
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Itoh Namiko
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Yamamoto Hiroyuki
Department Of Pharmacology Wakayama Medical University
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Yamamoto H
First Department Of Internal Medicine Sapporo Medical University
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Yamamoto Hiroyuki
Laboratory For Biopolymer Chemistry Department Of Polymer Science And Engineering Kyoto Institute Of
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