A Missense Mutation in Tomato mosaic virus L_<11>A-Fukushima Genome Determines Its Symptomless Systemic Infection of Tomato
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概要
- 論文の詳細を見る
The genome of an attenuated isolate L_<11>A-Fukushima (L_<11>A-F) of Tomato mosaic virus was cloned, its complete nucleotide sequence was determined, and full-length clone of L_<11>A-F was then assembled. Its transcripts systemically infeeted tomato without causing any symptom development. When a missense back mutation at the nucleotide correspondent to nucleotide 2350 of the genome was introduced into the clone, its transcripts produced distinct mosaic on the upper leaves of the inoculated tomato, and virus accumulation in the upper leaves increased about five times. The missense mutation in the L_<11>A-F genome was thus confirmed to be sufficient to attenuate its virulence on tomato. (Received June 10, 2002 ; Accepted October 30, 2002)
- 2002-12-25
著者
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Yamamoto H
Laboratory Of Plant Pathology Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Inoue Masayasu
Akita Prefectural University
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Ueda K
Ntt Atsugi‐shi Jpn
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Yamamoto Hideki
Akita Prefectural Agriculture Forestry And Fisheries Res. Center Akita Jpn
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ABE Takeshi
Akita Prefectural College of Agriculture
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UEDA Kenji
Akita Prefectural University
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MATSUMOTO Tsutomu
Akita Prefectural College of Agriculture
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Matsumoto T
Akita Prefectural College Of Agriculture
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