Characterization of Chondroitin/Dermatan Sulfate Proteoglycans Synthesized by Bovine Retinal Pericytes in Culture(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Biology)

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Pericytes associate with the outside of endothelial cells in microvessels. Previous studies have shown that these cells synthesize glycosaminoglycans (GAGs) but the nature of the core proteins to which these GAGs are attached is unknown. In the present study, cultured bovine retinal pericytes were metabolically labeled with [^3H]glucosamine, [^<35>S]sodium sulfate or ^<35>S-labeIed amino acids and the proteoglycans synthesized by these cells were purified by DEAE-Sephacel ion exchange and molecular sieve Sepharose CL-4B chromatography. Separated proteoglycans were digested with papain, heparitinase or chondroitin ABC lyase and the GAGs characterized by Sepharose CL-6B chromatography. Proteoglycans were also assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis before and after digestion with chondroitin ABC lyase. Pericytes predominantly synthesize and secrete chondroitin or dermatan sulfate proteoglycans (CS/DS PCs) rather than heparan sulfate proteoglycans (HSPGs). Two subclasses of CS/DS PCs are synthesized by pericytes; one is a high Mr subclass with high charge density. This subclass eluted at the void volume of a Sepharose CL-4B molecular sieve column, was susceptible to chondroitin ABC lyase, and contained core proteins of ca. 550 and 450 kD which were recognized by antibody to versican. The other major subclass eluted at a K_<av> ca. 0.45 on a Sepharose CL-4B molecular sieve column, was susceptible to chondroitin ABC lyase, and contained core proteins recognized by antibodies to either biglycan or decorin that separated as a broad band of ca. 50kDa in SDS-PAGE. A small amount of HSPG was also synthesized by these cells and could be separated from the CS/DS PCs by DEAE-Sephacel chromatography using a linear gradient of 0.1-0.7 M NaCl. Release of GAG chains by protease digestion indicated that the length of GAG chains was approximately M_r 45000 in biglycan and decorin, approximately M_r 48000 in the small amount of HSPGs and approximately M_r 66000 in versican. These proteoglycans resemble those synthesized by vascular smooth muscle cells but differ markedly from those synthesized by vascular endothelial cells.
社団法人日本薬学会の論文
2004-11-01

Characterization of Chondroitin/Dermatan Sulfate Proteoglycans Synthesized by Bovine Retinal Pericytes in Culture(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Biology)

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