Conjugation in Tetrahymena : Its Relation to Concanavalin A Receptor Distribution on the Cell Surface : Cell Biology

概要

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Cell surface events during conjugation of Tetrahymena thermophila were studied by electron microscopic examination of ferritin conjugated concanavalin A (F-Con A). Small amounts of ferritin particles (F-particles) were bound to the surface of cells in the nutritional and starved states, but there were no large clusters of F-particles. In regions where F-particles were scantly, the ectoplasmic layer (epiplasm) was directly under the plasma membrane and no alveoli were observed. In contrast, in cells during co-stimulation, large clusters of F-particles were seen on the presumptive junctional area (PJA) formed after the onset of co-stimulation between the complementary mating types, and on the side walls of ectoplasmic ridges of the oral apparatus. In regions of large clusters of F-particles, there was a thick, dense ectoplasmic layer under the plasma membrane and no alveoli were seen. In conjugants, F-particles were seen not only on the smooth PJA but also in zones of gaps in the junctional area between the two conjugants. These findings suggest that the Con A binding glycocalyx is anchored to the ectoplasmic layer, a kind of cytoskeleton, under the plasma membrane.
社団法人日本動物学会の論文
1988-04-15