The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region

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概要

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• The Pro-form (Pro-EndoPG I) of Stereum purpureum endopolygalacturonase I has a unique C-terminal region (pro-sequence) that is lacking in PGs of other origins. Mature EndoPG I purified from the culture filtrate of this fungus does not have the 44-amino-acid pro-sequence present in Pro-EndoPG I. We expressed Pro-EndoPG I in Escherichia coli and examined its activity. It was found that Pro-EndoPG I had no PG activity, but that PG activity was acquired after the degradation of part of the pro-sequence with V8 protease. These results suggest that the pro-sequence inactivates auto-PG activity. No similar characteristic has been reported for any glycoside hydrolase. We then constructed EndoPG I mutants and identified two Glu residues, E364 and E366, that were related to auto-inactivation. A test involving injection of the enzyme into apple trees showed that Pro-EndoPG I induced the same silver-leaf symptoms as mature EndoPG I.

• 2010-03-23

著者

• Ogawa Shun

  Faculty of Agriculture and Life Science, Hirosaki University

• Shimizu Tetsuya

  RIKEN, Harima Institute at Spring-8

• Kimura Tsubasa

  Faculty of Agriculture and Life Science, Hirosaki University

• UTOH Kazunori

  Faculty of Agriculture and Life Science, Hirosaki University

• OKUNO Toshikatsu

  Akita University of Nursing and Welfare

• MIYAIRI Kazuo

  Faculty of Agriculture and Life Science, Hirosaki University

• Miyairi Kazuo

  Faculty Of Agriculture And Life Science Hirosaki University

• Ogawa Shun

  Faculty Of Agriculture And Life Science Hirosaki University

• Shimizu Tetsuya

  Riken Spring-8 Center

• Utoh Kazunori

  Faculty Of Agriculture And Life Science Hirosaki University

• Kimura Tsubasa

  Faculty Of Agriculture And Life Science Hirosaki University

• Shimizu Tetsuya

  Riken

関連論文

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