The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region

スポンサーリンク

概要

• 論文の詳細を見る

• The Pro-form (Pro-EndoPG I) of Stereum purpureum endopolygalacturonase I has a unique C-terminal region (pro-sequence) that is lacking in PGs of other origins. Mature EndoPG I purified from the culture filtrate of this fungus does not have the 44-amino-acid pro-sequence present in Pro-EndoPG I. We expressed Pro-EndoPG I in Escherichia coli and examined its activity. It was found that Pro-EndoPG I had no PG activity, but that PG activity was acquired after the degradation of part of the pro-sequence with V8 protease. These results suggest that the pro-sequence inactivates auto-PG activity. No similar characteristic has been reported for any glycoside hydrolase. We then constructed EndoPG I mutants and identified two Glu residues, E364 and E366, that were related to auto-inactivation. A test involving injection of the enzyme into apple trees showed that Pro-EndoPG I induced the same silver-leaf symptoms as mature EndoPG I.

著者

• Ogawa Shun

  Faculty of Agriculture and Life Science, Hirosaki University

• Shimizu Tetsuya

  RIKEN, Harima Institute at Spring-8

• Kimura Tsubasa

  Faculty of Agriculture and Life Science, Hirosaki University

• UTOH Kazunori

  Faculty of Agriculture and Life Science, Hirosaki University

• OKUNO Toshikatsu

  Akita University of Nursing and Welfare

• MIYAIRI Kazuo

  Faculty of Agriculture and Life Science, Hirosaki University

関連論文

• The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region
• PA92 NOVEL PHYTOTOXINS FROM THE PATHOGENIC FUNGI ON APPLE
• Purification and Characterization of an Endopolygalacturonase from Physalospora piricola
• P-42 SYNTHETIC STUDIES OF OLIGOGALACTURONATE ANALOGUES AS MOLECULAR PROBES FOR endo-PG1
• β-Selective Glycosylation of 5-Thioglucopyranose Derivatives; Syntheses of β-(1→6) Linked 5'-Thioglucopyranosyl Disaccharides
• Characterization of an endopolygalacturonase Gene cppg1 from Phytopathogenic Fungus Chondrostereum purpureum
• Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum
• Cloning and Sequence Analysis of cDNA Encoding Endopolygalacturonase I from Stereum purpureum
• Novel Exopolygalacturonases Produced by Alternaria mali
• 2P024 紫外可視分光法によるタンパク質結晶の放射線損傷評価(蛋白質-構造,第48回日本生物物理学会年会)
• Synthesis of _D-Trigalacturonic Acid Methylglycoside and Conformational Comparison with Its Sulfur Analogue
• The Pro-Form of Stereum purpureum Endopolygalacturonase I Is Inactivated by a Pro-Sequence in the C-Terminal Region
• Cloning of the Gene Encoding an Endo-Acting Pectate Lyase from Streptomyces thermocarboxydus
• Glycosylation Status and Conformational Stability of Recombinant Stereum purpureum Endopolygalacturonase IVs Produced in an Aspergillus oryzae Expression System
• Active Suppression of EndoPG IV by Ligation of the Pro-Sequence from Stereum purpureum Pro-EndoPG I
• Isolation and Identification of Causal Constituents of Green Discoloration in Garlic Puree

もっと見る 閉じる

スポンサーリンク