Purification and Characterization of an Endopolygalacturonase from Physalospora piricola
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概要
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An endopolygalacturonase from Physalospora piricola was purified to apparent homogeneity by column chromatographies. The molecular weight was estimated to be 38,000 by SDS-PAGE, and the isoelectric point was around pH 7. 6. The amino acid composition was similar to those of endo PGs from other fungi. The enzyme was most active at pH 4.9 and stable from pH 5 to 7. Oligogalacturonic acids with a degree of polymerization higher than three were readily hydrolyzed by the enzyme, but trigalacturonic acid only slightly.
- 社団法人日本農芸化学会の論文
- 1994-10-23
著者
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MIYAIRI Kazuo
Faculty of Agriculture and Life Science, Hirosaki University
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Miyairi K
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
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MIYAIRI Kazuo
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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OKUNO Toshikatsu
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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Miyairi Kazuo
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
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Miyairi Kazuo
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Matsue Toshihide
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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Kagawa Osamu
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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Kutsuzawa Tomohiro
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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Kagawa Osamu
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Matsue Toshihide
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Kutsuzawa Tomohiro
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Okuno T
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Okuno Toshikatsu
Department of Agricultural Chemistry, Faculty of Agriculture, Hirosaki University
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