Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum
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概要
- 論文の詳細を見る
Three endopolygalacturonases (endoPG Ia, Ib, and Ic) were isolated from the culture filtrate of Stereum purpureum, the causative fungus of apple silver-leaf disease. Their properties, including specific activities, optimum pHs, thermal stabilities, and kinetic parameters (K_m and V_<max>) were compared. Their properties were very similar to one another except for the substrate specificity and relative molecular mass. The sugar chains of endoPG Is were released by hydrazinolysis, and one major sugar chain common to endoPG Is was isolated. The pyridylamino sugar was characterized by a two-dimensional mapping method using HPLC, and identified as a high mannose type N-linked sugar chain, Manα1-6 (Manα1-3) Manα1-6 (Manα1-3) Manβ1-4GlcNAcβ1-4 GlcNAc (designated as M5.1). Observation of the course of Western blot analysis for the proteins from the culture filtrate with endoPG I antibodies showed that the fungus secreted three endoPG Is into the culture broth during the growing period.
- 社団法人日本農芸化学会の論文
- 1998-05-23
著者
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MIYAIRI Kazuo
Faculty of Agriculture and Life Science, Hirosaki University
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Miyairi K
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
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MIYAIRI Kazuo
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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OKUNO Toshikatsu
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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Miyairi Kazuo
Fac. Of Agriculture And Life Sci. Hirosaki Univ.
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HASUI Yuji
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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FUKUI Yohko
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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KIKUCHI Junko
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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KATO Nozomu
Department of Bioresources Science, Faculty of Agriculture, Hirosaki University
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Fukui Y
Research Center For Environmental Genomics Kobe University
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Hasui Y
Osaka Municipal Technical Res. Inst. Osaka Jpn
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Kato Nozomu
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Kikuchi Junko
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Kikuchi Junko
Department Of Bioresources Science Faculty Of Agriculture Hirosaki University
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Okuno T
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Fukui Yuko
Institute For Fundamental Research Suntory Ltd. (sunbor)
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Hasui Yuji
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Okuno Toshikatsu
Department of Agricultural Chemistry, Faculty of Agriculture, Hirosaki University
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