Inportance of Phosphatidylinositol 3-Phosphate in Sporulation of Schizosaccharomyces pombe(Biochemistry & Molecular Biology)

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概要

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• In Schizosaccharomyces pombe, Pik3p phosphorylates phosphatidylinositol (PI) to produce PI 3-P, which is further phosphorylated by Stel2p to yield PI 3, 5-P_2. Pik3p is required for both conjugation and sporulation. To test which of PI 3-P and PI 3, 5-P_2 is required for sporulation, diploid cells defective in production of PI 3, 5-P_2 were used. They underwent sporulation almost normally provided that the osmotic pressure of the medium was controlled, suggesting that not PI 3, 5-P_2 but PI 3-P was important. Electron microscopic analysis confirmed normal sporulation in the absence of PI 3, 5-P_2 although the forespore membrane was found to be less dense in these cells.

• 社団法人日本農芸化学会の論文
• 2003-05-23

著者

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• Nakamura Yoko

  Laboratory Of Biological Chemistry Graduate School Of Agricultural And Life Science University Of To

• HIRATA Aiko

  Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

• FUKUI Yasuhisa

  Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agr

• Nakamura Y

  Department Of Biofunctional Chemistry Division Of Bioscience Graduate School Of Natural Science And

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• ONISHI Masayuki

  Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of

• KOGA Takako

  Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of

• Fukui Y

  Research Center For Environmental Genomics Kobe University

• Hirata Aiko

  Department Of Integrated Biosciences Graduate School Of Frontier Science University Of Tokyo

• Onishi Miki

  Laboratory Of Biological Chemistry Graduate School Of Agricultural And Life Science University Of To

• Fukui Yasuhisa

  Laboratory Of Biological Chemistry Division Of Applied Biological Chemistry Graduate School Of Agric

• Fukui Yuko

  Institute For Fundamental Research Suntory Ltd. (sunbor)

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