Isolation and Characterization of a Novel Endo-β-galactofuranosidase from Bacillus sp.

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A soil bacterium capable of growing on a polysaccharide containing β(1→6)galactofuranoside residues derived from the acidic polysaccharide of Fusarium sp. as a carbon source has been isolated. From various bacteriological characteristics, the organism was identified as a Bacillus sp. The bacterium produced β-galactofuranosidase inductively in the culture media. The most effective inducer for the β-galacto-furanosidase production was a polysaccharide containing β(1→5) or β(1→6)-linked galactofuranoside residues, but gum arabic, gum guar, gum ghati, arabinogalactam, araban, and pectic acid did not induce the enzyme. The enzyme had three different molecular weight forms. The low molecular-weight form was purified by a combination of Toyopearl HW-55 and DEAE-Toyopearl 650S column chromatographies, and preparative polyacrylamide gel electrophoresis. The molecular weight of the enzyme was estimated to be 67, 000 by SDS-polyacrylamide gel electrophoresis. The enzyme was most active at pH 6 and 37oC, and was stable between pH 4 to 8 at 5oC. The action of the enzyme was inhibited by the addition of Cd2 +, Co2+, Hg2+, Zn2+, iodoacetic acid, and EDTA. The purified enzyme cleaved β(1→5) and β(1→6)-linked galactofuranosyl chains. Based upon the mode of liberation of galactofuranosyl residues from pyridylamino-β(1→6)-linked galactofuranoside oligomers, the enzyme can be classified as an endo-β-galactofuranosidase that randomly hydrolyzes the linkage.
社団法人日本農芸化学会の論文
1995-10-23

Isolation and Characterization of a Novel Endo-β-galactofuranosidase from Bacillus sp.

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