Functional Expression of Schizosaccharomyces pombe Vba2p in the Vacuolar Membrane of Saccharomyces cerevisiae
スポンサーリンク
概要
- 論文の詳細を見る
A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.
著者
-
Iwaki Tomoko
Department Of Biological Resources Faculty Of Agriculture Ehime University
-
SUGIMOTO Naoko
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
SEKITO Takayuki
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
KAKINUMA Yoshimi
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
-
Takegawa Kaoru
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
-
AKIYAMA Koichi
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
PONGCHAROEN Pongsanat
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
KAWANO-KAWADA Miyuki
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
-
IWAKI Tomoko
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
関連論文
- Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus
- Atg22p, a Vacuolar Membrane Protein Involved in the Amino Acid Compartmentalization of Schizosaccharomyces pombe
- Vba2p, A Vacuolar Membrane Protein Involved in Basic Amino Acid Transport in Schizosaccharomyces pombe
- An α-Amylase Homologue, aah3, Encodes a GPI-Anchored Membrane Protein Required for Cell Wall Integrity and Morphogenesis in Schizosaccharomyces pombe
- Mitochondrial ABC Transporter Atm1p Is Required for Protection against Oxidative Stress and Vacuolar Functions in Schizosaccharomyces pombe
- Vesicle-mediated Protein Transport Pathways to the Vacuole in Schizosaccharomyces pombe
- Production and Characterization of Extracellular Uronic Acid-Containing Glycoproteins from Fusarium oxysporum
- Isolation and Characterization of a Glycosylation Mutant from Schizosaccharomyces pombe
- Degradation of β1→6 Galactofuranoside Linkages in the Polysaccharide of Fusarium sp. M7-1 by Endo-β-galactofuranosidase from Bacillus sp.
- Isolation and Identification of a Choline-linked Mannobiose in the Glycoproteins of Fusarium sp. M7-1
- Isolation and Characterization of a Novel Endo-β-galactofuranosidase from Bacillus sp.
- Isolation and Identification of Novel Acidic Oligosaccharides Derived from Glycoproteinsof Fusarium sp. M7-1
- Preparation of β(1→6)-Linked Galactofuranoside Oligomers from the Acidic Polysaccharide of Fusarium sp. M7-1
- Degradation of Unsaturated Polysaccharide Derived from Acidic Polysaccharide of Fusarium sp. : M7-1 by a Bacterium Isolated from Soil
- Assessing the efficacy of famotidine and rebamipide in the treatment of gastric mucosal lesions in patients receiving long-term NSAID therapy (FORCE-famotidine or rebamipide in comparison by endoscopy)
- Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Microbiology & Fermentation Industry)
- Effect of Yeast Extract on Endo-β-N-acetylglucosaminidase Production by a Flavobacterium sp.(Microbiology & Fermentation Industry)
- Vacuolar Amino Acid Transporter Avt5p Is Responsible for Lithium Uptake in Schizosaccharomyces pombe
- 3P-134 腸内連鎖球菌V型ATPaseの膜内在性サブユニット(NtpI)の発現と精製(分子モーター,第47回日本生物物理学会年会)
- 2P-188 腸球菌ナトリウム輸送性V型ATPaseの膜内在性ローターリングのエンジニアリング(分子モーター(2),第46回日本生物物理学会年会)
- 1P-144 腸内連鎖球菌V型ATPaseの外周ストークのサブユニット構成とその溶液構造(分子モーター(1),第46回日本生物物理学会年会)
- A Set of loxP Marker Cassettes for Cre-mediated Multiple Gene Disruption in Schizosaccharomyces pombe
- Characterization of the Na^+ -ATPase Gene(ZENAI)from the Salt-Tolerant Yeast Zygosaccharomyces rouxii
- Heavy oil fractions induce negative influences on mouse immune system
- Tributyltin induces Yca1p-dependent cell death of yeast Saccharomyces cerevisiae
- Severe abnormalities in the reproductive organs of mice caused by chemical substances contained in heavy oil
- Tributyltin sensitivity of vacuolar-type Na^+-transporting ATPase from Enterococcus hirae
- Characterization of Endo-β-N-acetylglucosaminidase from Alkaliphilic Bacillus halodurans C-125
- Identification of Amino Acid Residues Essential for the Substrate Specificity of Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Biochemistry & Molecular Biology)
- Plate Assay for Endo-β-N-Acetylglucosaminidase Activity Using a Chromogenic Substrate Synthesized by Transglycosylation with Arthrobacter Endo-β-N-Acetylglucosaminidase
- Primary Structure of an O-Linked Sugar Chain Derived from Glucose Oxidase of Aspergillus niger(Biological Chemistry)
- The Release of Oligosaccharides from Glycoproteins by Endo-β-N-Acetylglucosaminidase of Flavobacterium sp.
- Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-β-N-acetylglu-cosaminidase from Flavo-bacterium sp.
- Loss of a GPI-Anchored Membrane Protein Aah3p Causes a Defect in Vacuolar Protein Sorting in Schizosaccharomyces pombe
- Deletion Analysis of the Subunit Genes of V-Type Na^+-ATPase from Enterococcus hirae
- Identification of Nucleotide Binding Sites in V-Type Na^+-ATPase from Enterococcus hirae
- Indispensable Glutamic Acid Residue-139 of NtpK Proteolipid in the Reaction of Vacuolar Na^+-Translocating ATPase in Enterococcus hirae
- Properties of the V_0 V_1 Na^+-ATPase from Enterococcus hirae and Its V_0 Moiety
- Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides(Microbiology & Fermentation Industry)
- Effect of Deglycosylation of Polymannose Chains on the Properties of Yeast External Invertase
- Identification of O-α-D-Glucopyranosyluronate-(1→2)-D-galactose Isolated from the Acidic Polysaccharide of Fusarium sp. M7-1(Microbiology & Fermentation Industry)
- Purification and Properties of a Low-Molecular-Weight α-Mannosidase from Cellulomonas sp.
- β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp.(Microbiology & Fermentation Industry)
- Action of β-Mannosidases on O-β-D-Mannopyranosyl-(1→2)-D-mannose(Biological Chemistry)
- Synthesis of Bioactive Peptide Glycosylated at Two Sites
- Chemo-Enzymatic Synthesis of a Calcitonin Derivative Containing a High-Mannose Type Oligosaccharide by Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae
- Isolation of Enterococcus hirae Mutant Deficient in Low-affinity Potassium Uptake at Alkaline pH(Microbiology & Fermentation Technology)
- Isolation of Suppressor Mutants of Phosphatidylinositol 3-Phosphate 5-kinase Deficient Cells in Schizosaccharomyces pombe(Microbiology & Fermentation Technology)
- Functional Conservation between Fission Yeast moc1/sds23 and Its Two Orthologs, Budding Yeast SDS23 and SDS24, and Phenotypic Differences in Their Disruptants
- エンドグリコシダーゼの糖転移活性とその応用
- Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 分裂酵毋 Schizosaccharomyces pombe におけるガラクトース鎖付加機構と生理的役割
- Neurohistology of the sinus tarsi and sinus tarsi syndrome
- Nucleotide-Binding Sites in V-Type Na^+-ATPase from Enterococcus hirae
- Activation of Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae by Various Sugars
- New insights into galactose metabolism by Schizosaccharomyces pombe: Isolation and characterization of a galactose-assimilating mutant(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- NapA Na^+/H^+ Antiporter as a Sodium Extrusion System Supplementary to the Vacuolar Na^+-ATPase in Enterococcus hirae
- The och1 Mutant of Schizosaccharomyces pombe Produces Galactosylated Core Structures of N-Linked Oligosaccharides
- Transfer of High-Mannose-Type Oligosaccharides to Disaccharides by Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae
- Purification and Properties of Chondroitinase Produced by a Bacterium Isolated from Soil
- PXA Domain-Containing Protein Pxa1 Is Required for Normal Vacuole Function and Morphology in Schizosaccharomyces pombe
- Loss of a GPI-Anchored Membrane Protein Aah3p Causes a Defect in Vacuolar Protein Sorting in Schizosaccharomyces pombe
- Vba2p, A Vacuolar Membrane Protein Involved in Basic Amino Acid Transport in Schizosaccharomyces pombe
- Galactinol Synthase Gene of Coptis japonica Is Involved in Berberine Tolerance
- Vacuolar Amino Acid Transporter Avt5p Is Responsible for Lithium Uptake in Schizosaccharomyces pombe
- Atg22p, a Vacuolar Membrane Protein Involved in the Amino Acid Compartmentalization of Schizosaccharomyces pombe
- Inhibition of myotube formation by paraquat in the myoblast cell line C2C12
- A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling
- Nystatin Effects on Vacuolar Function in Saccharomyces cerevisiae
- The Presence of Trehalose-containing Oligosaccharides in Yeast Extract
- Characterization of Two Genes Encoding Putative Cysteine Synthase Required for Cysteine Biosynthesis in Schizosaccharomyces pombe
- The secondary structure and phylogenetic relationship deduced from complete nucleotide sequence of mitochondrial small subunit rRNA in yeast Hansenula wingei
- Disruption of Ion Homeostasis by Verrucosin and a Related Compound
- New insights into galactose metabolism by Schizosaccharomyces pombe : Isolation and characterization of a galactose-assimilating mutant
- Disruption of the plr1^+ Gene Encoding Pyridoxal Reductase of Schizosaccharomyces pombe
- Enrichment and Characterization of a Trichloroethene-Dechlorinating Consortium Containing Multiple "Dehalococcoides" Strains
- Heavy oil fraction induces the dysplastic sperm in male mouse
- Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Galactose-Specific Recognition System in the Fission Yeast Schizosaccharomyces pombe
- CUE Domain-Containing Protein Vps901 Is Required for Vacuolar Protein Transport in Schizosaccharomyces pombe
- Genome-wide association study to identify genetic variants present in Japanese patients harboring intracranial aneurysms
- CUE Domain-Containing Protein Vps901 Is Required for Vacuolar Protein Transport in Schizosaccharomyces pombe
- 分裂酵母 Schizosaccharomyces pombe におけるガラクトース特異的な認識システム
- Functional Expression of Schizosaccharomyces pombe Vba2p in the Vacuolar Membrane of Saccharomyces cerevisiae
- Purification and Characterization of Endo-β-N-acetylglucosaminidase from a Flavobacterium sp.
- Effect of Protease Digestion on the Activity of Sugardepleted Enzymes Prepared with Endo-β-N-acetylglucosaminidase from Flavobacterium sp.