Functional Conservation between Fission Yeast moc1/sds23 and Its Two Orthologs, Budding Yeast SDS23 and SDS24, and Phenotypic Differences in Their Disruptants
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概要
- 論文の詳細を見る
The moc1/sds23 gene was isolated to induce sexual development of a sterile strain due to overexpression of adenylate cyclase in Schizosaccharomyces pombe. Here, we studied the functional conservation between moc1/sds23 and its two orthologs SDS23 and SDS24 in Saccharomyces cerevisiae. We observed that the temperature sensitivity, salt tolerance, cell morphology, and sterility of the Δmoc1 mutant in S. pombe were recovered by expressing either S. cerevisiae SDS23 or SDS24. We found that deletion of both SDS23 and SDS24 resulted in the production of a large vacuole that was reversed by the expression of S. pombe moc1/sds23. In these ways we found that S. pombe Moc1/Sds23 and S. cerevisiae SDS23p or SDS24p are functional homologs. In addition we found that the Δsds23 Δsds24 diploid strain reduces cell separation in forming pseudohyphal-like growth in S. cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
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TAKEGAWA Kaoru
Department of Life Science, Faculty of Agriculture, Kagawa University
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Takegawa Kaoru
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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ISHIKURA Yasue
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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Ishikura Yasue
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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GOLDAR Md.
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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NISHIE Toshikazu
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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FUKUDA Toshifumi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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Nishie Toshikazu
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Goldar Md.
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Fukuda Toshifumi
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Ishikura Yasue
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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