Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
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概要
- 論文の詳細を見る
We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were β-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).
- 社団法人 日本農芸化学会の論文
- 2009-11-23
著者
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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Ishiguro Sumie
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Ishiguro S
Nagoya Univ. Nagoya Jpn
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Ishiguro Sumie
Dept. Botany Grad. Sch. Science Kyoto Univ.
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Ishiguro Sumie
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Takahashi N
Plant Functions Laboratory Riken
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Nakao Akihide
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Kimura Tetsuya
Department Of Clinical Retrovirology And Infectious Diseases Center For Aids Research Kumamoto Unive
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Kawamukai Makoto
Department Of Natural Resources Faculty Of Agriculture Shimane University
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kimura Tetsuya
Dep. Of Sustainable Resource Sci. Graduate School Of Bioresources Mie Univ.
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Nakamura Shinya
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Nakao Akihide
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Takahashi Nobuyuki
Hokkaido University Of Education
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Nakano Takeshi
Plant Functions Lab. The Inst. Of Physical And Chemical Res.
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Nakano Takeshi
Plant Functions Lab.
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Nakamura Shinya
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Okayama University
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Nakamura Shinya
Department Of Chemistry University Of Tsukuba
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Takahashi Nobuyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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Kimura Tetsuya
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
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Takahashi Noriko
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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NAKAGAWA Tsuyoshi
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
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