Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
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概要
- 論文の詳細を見る
We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.
著者
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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Mano Shoji
Department of Cell Biology, National Institute for Basic Biology
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Tanaka Yuji
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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OHNISHI Masato
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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NAKAMORI Chihiro
Department of Cell Biology, National Institute for Basic Biology
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ARAKI Masami
Department of Cell Biology, National Institute for Basic Biology
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NIWA Tomoko
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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NISHIMURA Mikio
Department of Cell Biology, National Institute for Basic Biology
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KAMINAKA Hironori
Department of Bioresource Sciences, Faculty of Agriculture, Tottori University
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NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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SATO Yutaka
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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