Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Two tonoplast intrinsic proteins (TIP) of pumpkin seeds, pMP23 and MP28, were expressed in yeast cells under control of the GAL1 promoter, and the subcellular localization of the proteins was analyzed. The pMP23 and MP28 stably accumulated in the yeast vacuolar membrane when the proteins were expressed in the proteinase A-deficient strain (pep4), which lacks the activities of vacuolar proteases. However, pMP23 and MP28 did not accumulate in the wild-type strain; the expressed pMP23 and MP28 were degraded in a proteinase A-dependent manner. These results indicate that pMP23 and MP28 are transported to the vacuolar membrane when expressed in yeast.

• 日本植物生理学会の論文

著者

• NISHIMURA Mikio

  Department of Cell Biology, National Institute for Basic Biology

• INOUE Kaori

  Department of Diagnostic Pathology, Kochi Red Cross Hospital

• Nishimura Mikio

  Department Of Cell Biology National Institute For Basic Biology

• Nishimura Mikio

  Dept. Cell Biol. Nat. Inst. Basic. Biol.

• Nishimura Mikio

  Department Of Cell Biology National Institute For Basic Biology:department Of Molecular Biomechanics

• Wada Yoh

  Department of Biology, Graduate School of Arts and Sciences, University of Tokyo

• Hara-Nishimura lkuko

  Department of Cell Biology, National Institute for Basic Biology

• Inoue Kaori

  Department Of Cardiology Nagoya Memorial Hospital

• Inoue Kaori

  Department Of Cell Biology National Institute For Basic Biology:department Of Molecular Biomechanics

• Inoue K

  Department Of Cell Biology National Institute For Basic Biology:department Of Molecular Biomechanics

• Nishimura M

  Dept. Cell Biol. Nat. Inst. Basic Biol.

• Hara-nishimura Lkuko

  Department Of Cell Biology National Institute For Basic Biology:department Of Molecular Biomechanics

• Wada Yoh

  Department Of Biology Graduate School Of Arts And Sciences University Of Tokyo

• Wada Yoh

  Department Of Biology College Of Arts And Sciences University Of Tokyo

関連論文

• Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
• Diagnostic pitfall on the histological spectrum of adult-onset renal carcinoma associated with Xp11.2 translocations/TFE3 gene fusions
• DPE-053 Diagnostic Accuracy of Coronary Artery Stenosis Using Combination of Computed Tomography Angiography and Adenosine Stress Myocardial Perfusion Imaging(DPE09,CT/MRI (Coronary/Vascular) (I),Digital Poster Session (English),The 73rd Annual Scientific
• OE-313 Impact of Impaired Myocardial Perfusion and Fatty Acid Metabolism on Ventricular Remodeling following Acute Myocardial Infarction(OE53,Nuclear Cardiology (Coronary, Myocardium) 2 (I),Oral Presentation (English),The 73rd Annual Scientific Meeting of
• Immunohistochemical application of D2-40 as basal cell marker in evaluating atypical small acinar proliferation of initial routine prostatic needle biopsy materials
• Cytosolic Aconitase Participates in the Glyoxylate Cycle in Etiolated Pumpkin Cotyledons : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
• Spatial Patterns of Sucrose-Inducible and Polygalacturonic Acid-Inducible Expression of Genes That Encode Sporamin and β-Amylase in Sweet Potato : GENE STRUCTURE AND EXPRESSION
• Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display
• Molecular Cloning of a Defense-Response-Related Cytochrome P450 Gene from Tobacco
• The Involvement of Tonoplast Proton Pumps and Na^+(K^+)/H^+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
• Different Changes in the Expression of Multiple Kinds of Tight-Junction Proteins during Ischemia-Reperfusion Injury of the Rat Ileum
• 99mTc-MIBI Washout Rate in Patients with Sarcoidosis(Nuclear Cardiology 7 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
• A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
• PE-323 Coronary CTA can Predict Slow Flow after PCI(CT/DSA-07, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Atherosclerotic Plaque Characterization by 0.5-mm-Slice Multislice Computed Tomographic Imaging : Comparison With Intravascular Ultrasound
• PJ-748 Comparison of Atherosclerotic Plaque Characteristics by IVUS and Multislice CT(CT/DSA-9 (I) PJ128,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• PE-098 Assessment of Coronary Plaque Volume Measurement Using MSCT on Phantom Study : What Factors Affecting on Measurement?(CT/DSA-3 (I) PE17,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
• Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells
• Detection and Characterization of a Vacuolar Protein(VP24) in Anthocyanin Producing Cells of Sweet Potato in Suspension Culture : PROTEINS, ENZYMES AND METABOLISM : STRUCTURE AND FUNCTION OF CELLS
• AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells
• An ER-Localized Form of PV72, a Seed-Specific Vacuolar Sorting Receptor, Interferes the Transport of an NPIR-Containing Proteinase in Arabidopsis Leaves
• Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana
• The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis
• A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
• Proteomic Analysis of Leaf Peroxisomal proteins in Greening Cotyledons of Arabidopsis thaliana
• Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana
• Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP : Dynamic Morphology and Actin-Dependent Movement
• Activation of Arabidopsis Vacuolar Processing Enzyme by Self-Catalytic Removal of an Auto-Inhibitory Domain of the C-Terminal Propeptide
• Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid β-Oxidation
• Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons
• Pumpkin Peroxisomal Ascorbate Peroxidase is Localized on Peroxisomal Membranes and Unknown Membranous Structures
• THE ANALYSIS OF THE TRANSPORT PATHWAY TO VACUOLES DIRECTLY FROM ER
• BINDING ANALYSIS OF A VACUOLAR SORTING RECEPTOR, PV72
• A NOVEL VACUOLAR MEMBRANE PROTEIN, MP73,IS TRANSPORTED TO VACUOLES VIA PAC VESICLES
• THE PROCESSING MECHANISM AND THE PHYSIOLOGICAL FUNCTION OF VEGETATIVE TYPE VACUOLAR PROCESSING ENZYME IN HIGHER PLANTS
• Distribution and characterization of peroxisomes in Arabidopsis thaliana by visualization with GFP
• Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana
• Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
• Expression and Localization of a 36-kDa Peptide Derived from a 24-kDa Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Sweet Potato Cells in Suspension Culture
• Tissue and Intracellular Localization of Indican and the Purification and Characterization of Indican Synthase from Indigo Plants
• A Novel Isoenzyme of Ascorbate Peroxidase Localized on Glyoxysomal and Leaf Peroxisomal Membranes in Pumpkin : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
• Development of Microbody Membrane Proteins during the Transformation of Glyoxysomes to Leaf Peroxisomes in Pumpkin Cotyledons : MEMBRANES AND BIOENERGETICS : STRUCTURE AND FUNCTION OF CELLS
• Cloning and Sequencing of cDNA for Glycolate Oxidase from Pumpkin Cotyledons and Northern Blot Analysis
• Characterization of Intravacuolar Pigmented Structures in Anthocyanin-Containing Cells of Sweet Potato Suspension Cultures
• The Sequence and Expression of the γ-VPE Gene, One Member of a Family of Three Genes for Vacuolar Processing Enzymes in Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
• ISOLATION AND CHARACTERIZATION OF ARABIDOPSIS MUTANTS THAT HAVE DEFECTS IN THE PROCESSING OF SEED STORAGE PROTEINS
• THE LOCALIZATION OF PUMPKIN PV72,A SORTING RECEPTOR FOR VACUOLAR PROTEINS
• Characterization of a putative vacuolar sorting receptor, PV72,in developing pumpkin seeds
• TRANSPORT PATHWAYS FOR SEED STORAGE PROTEINS TO THE VACUOLES OF HIGHER PLANTS
• ANALYSIS OF THE VACUOLAR TARGETING SIGNAL OF PUMPKIN PRO2S ALBUMIN
• MOLECULAR CHARACTERIZATION OF ARABIDOPSIS CO-CHAPERONINS.
• MULTIPLE FUNCTIONAL PROTEINS ARE PRODUCED BY CLEAVING ASN-GLN BONDS OF A SINGLE PRECURSOR BY VACUOLAR PROCESSING ENZYME
• A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor
• A Rapid Increase in the Level of Binding Protein (BiP) is Accompanied bySynthesis and Degradation of Storage Proteins in Pumpkin Cotyledons
• A NOVEL MEMBRANE PROTEIN, PMP38,IS LOCALIZED ON PLANT GLYOXYSOMAL MEMBRANES
• INTRACELLULAR TRANSPORT OF A LEAF PEROXISOMAL 27-kDa PROTEIN FROM Arabidopsis thaliana
• Microbody Defective Mutants of Arabidopsis
• MOLECULAR MECHANISM FOR TARGETING OF PLANT CATALASE TO MICROBODIES
• Hydroxypyruvate Reductase with a Carboxy-Terminal Targeting Signal to Microbodies is Expressed in Arabidopsis
• cDNA Cloning and Expression of a Gene for Isocitrate Lyase in Pumpkin Cotyledons
• Functional Transformation of Microbodies in Higher Plant Cells
• Functional Classification of Arabidopsis Peroxisome Biogenesis Factors Proposed from Analyses of Knockdown Mutants
• Oligomeric Proteins Containing N-Terminal Targeting Signals are Imported into Peroxisomes in Transgenic Arabidopsis
• Accumulation of a Fusion Protein Containing 2S Albumin Induces Novel Vesicles in Vegetative Cells of Arabidopsis
• ASCORBATE PEROXIDASE THAT IS LOCALIZED IN MICROBODY MEMBRANES
• CHARACTERIZATION OF VEGETATIVE-TYPE VACUOLAR PROCESSING ENZYME of Arabidopsis
• ARABIDOPSIS ped2 MUTANT ACCUMULATES PRECURSOR WITH HIGH MOLECULAR MASS FOR PTS2-TYPE PEROXISOMAL PROTEINS
• PURIFICATION AND CHARACTERIZATION OF A PEROXISOMAL SHORT-CHAIN ACYL-COA OXIDASE FROM PUMPKIN
• MOLECULAR CHARACTERIZATION OF ACYL-CoA OXIDASE IN PUMPKIN
• ARABIDOPSIS ped1 MUTANT THAT HAS DEFECT IN THIOLASE GENE
• Glyoxysomal Malate Dehydrogenase in Pumpkin : Cloning of a cDNA and Functional Analysis of Its Presequence
• Changes in Targeting Efficiencies of Proteins to Plant Microbodies Caused by Amino Acid Substitutions in the Carboxy-terminal Tripeptide
• Cryptochromes and Phytochromes Synergistically Regulate Arabidopsis Root Greening under Blue Light
• Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation
• Behavior of Vacuoles during Microspore and Pollen Development in Arabidopsis thaliana
• Development of Enzymes of the Glyoxylate Cycle during Senescence of Pumpkin Cotyledons
• Glyoxylate Cycle Enzymes in Peroxisomes Isolated from Petals of Pumpkin (Cucurbita sp.) during Senescence
• Arabidopsis MUTANTS THAT HAVE DEFECTS ON GLYOXYSOMAL FUNCTIONS
• A Vacuolar Processing Enzyme in Maturing and Germinating Seeds : Its Distribution and Associated Changes during Development
• CHARACTERIZATION OF A PROTEIN COMPONENT, DV100, OF THE DENSE VESICLES FROM MATURING PUMPKIN SEEDS
• Small GTP-Binding Proteins are Associated with the Vesicles That are Targeted to Vacuoles in Developing Pumpkin Cotyledons : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
• Ectopic Expression of an Esterase, Which is a Candidate for the Unidentified Plant Cutinase, Causes Cuticular Defects in Arabidopsis thaliana
• Suppression of Peroxisome Biogenesis Factor 10 Reduces Cuticular Wax Accumulation by Disrupting the ER Network in Arabidopsis thaliana
• Constitutive and Inducible ER Bodies of Arabidopsis thaliana Accumulate Distinct β-Glucosidases
• Arabidopsis VPS35, a Retromer Component, is Required for Vacuolar Protein Sorting and Involved in Plant Growth and Leaf Senescence
• RECOGNITION OF THE N-TERMINAL TARGETING SIGNAL OF GLYOXYSOMAL CITRATE SYNTHASE PRECURSOR IN TRANSGENIC ARABIDOPSIS
• cDNA CLONING AND EXPRESSION OF A GENE FOR THE ENZYMES OF β-OXIDATION CYCLE IN PUMPKIN COTYLEDONS
• THE N-TERMINAL REGION OF GLYOXYSOMAL MALATE DEHYDROGENASE FROM PUMPKIN FUNCTION IN TARGETING TO MICROBODIES
• Plant Catalase is Imported into Peroxisomes by Pex5p but is Distinct from Typical PTS1 Import
• Vacuolar Processing Enzyme of Soybean That Converts Proproteins to the Corresponding Mature Forms : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
• Antagonistic Jacalin-Related Lectins Regulate the Size of ER Body-Type β-Glucosidase Complexes in Arabidopsis thaliana
• Proteomic Analysis of Highly Purified Peroxisomes from Etiolated Soybean Cotyledons
• Frontiers of Research on Organelle Differentiation
• Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics
• Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
• cDNA Cloning of Thylakoid-Bound Ascorbate Peroxidase in Pumpkin and Its Characterization
• A Defect of Peroxisomal Membrane Protein 38 Causes Enlargement of Peroxisomes
• Loss of XRN4 Function Can Trigger Cosuppression in a Sequence-Dependent Manner

もっと見る 閉じる

スポンサーリンク