Tissue and Intracellular Localization of Indican and the Purification and Characterization of Indican Synthase from Indigo Plants

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概要

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• Indican (indoxyl β-D-glucoside) was found to accumulate only in green leaves of the indigo plant, and not in any other tissues. Comparisons of the indican content of protoplasts and vacuoles showed that indican was stored only in the vacuole of the cell. Indican content appeared and increased with the appearence and growth of leaves. In mature plants, the younger leaves contained larger amounts of indican than the older ones. Cell extracts of young leaves of indigo plant catalyzed the synthesis of indican from UDP-glucose and indoxyl. Indican synthase was extracted and purified from young leaves. The enzyme was separated into two fractions by anion-exchange chromatography. The enzyme in the fraction which was eluted by 0.1 M NaCl had a molecular weight of 53, 000 by SDS-PAGE. Optimum pH of the enzyme was at about 10.0, indicating that the enzyme is likely localized in a different intracellular compartment from that of indican storage. The enzyme showed normal Michaelis-Menten Kinetics and a K_m value of 0.13 mM for UDP-glucose.

• 日本植物生理学会の論文

著者

• NISHIMURA Mikio

  Department of Cell Biology, National Institute for Basic Biology

• NISHIMURA Osamu

  Genome Resource and Analysis Subunit, RIKEN Center for Developmental Biology

• Minami Y

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Matsubara Hiroshi

  Department of Respirology, Graduate School of Medicine, Chiba University

• Miyamoto H

  Hitachi Ltd. Tokyo Jpn

• MINAMI Yoshiko

  Department of Biochemistry, Faculty of Science, Okayama University of Science

• Nishimura M

  Dep. Of Cell Biology National Inst. For Basic Biology

• Nishimura Mikio

  Department Of Cell Biology National Institute For Basic Biology

• Nishimura Mikio

  Dept. Cell Biol. Nat. Inst. Basic. Biol.

• Hara-Nishimura Ikuko

  Department of Botany, Graduate School of Science, Kyoto University

• Nishimura Osamu

  Department of Electronics and Computer Science

• HARA(NISHIMURA) Ikuko

  Department of Botany, Graduate School of Science, Kyoto University

• Hara-nishimura Ikuko

  Dept. Cell Biol. Nat. Inst. Basic. Biol.

• Hara Ikuko

  Department Of Botany Graduate School Of Science Kyoto University

• Hara-nishimura Ikuko

  Department Of Botany Graduate School Of Science Kyoto University

• MATSUMOTO Hitoshi

  Wireless Research Laboratory, Matsushita Electric Industrial Co., Ltd.

• Matsubara Hiroshi

  Department Of Dermatology Graduate School Of Medicine Chiba University

• Matsumoto Hiroshige

  Institute On Industrial Science University Of Tokyo

• Matsubara H

  Department Of Biochemistry Faculty Of Science Okayama University Of Science

• Matsubara H

  Development Department Dainippon Screen Manufacturing Co. Ltd.

• Minami Yoshiko

  Department Of Biochemistry Faculty Of Science Okayama University Of Science

• Matsumoto H

  Department Of Earth And Space Science Graduate School Of Science Osaka University

• Minami Y

  Okayama Univ. Sci. Okayama

• NISHIMURA Osamu

  RIKEN Center for Developmental Biology

• Nishimura Osamu

  Genome Resource And Analysis Subunit Riken Center For Developmental Biology

• Nishimura Osamu

  Department Of Earth Science And Astronomy College Of Arts And Sciences The University Of Tokyo

• Matsubara Hiroshi

  Department Of Biology Faculty Of Science Osaka University

• Matsubara Hiroshi

  Deparlment Of Internal Medicine School Of Medicine Kyoto University

• NISHIMURA Osamu

  Department of Civil and Environmental Engineering, Tohoku University, Japan

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