Changes in Targeting Efficiencies of Proteins to Plant Microbodies Caused by Amino Acid Substitutions in the Carboxy-terminal Tripeptide
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概要
- 論文の詳細を見る
It has been demonstrated that the carboxyl terminus of microbody enzymes functions as a targeting signal to microbodies in higher plants. We have examined an ability of 24 carboxy-terminal amino acid sequences to facilitate the transport of a cytosolic passenger protein, β-glucuronidase, into microbodies in green cotyledonary cells of transgenic Arabidopsis. Immunoelectron microscopic analysis revealed that carboxy-terminal tripeptide sequences of the form [C/A/S/P]-[K/R]-[I/L/M] function as a microbodytargeting signal, although tripeptides with proline at the first amino acid position and isoleucine at the carboxyl terminus show weak targeting efficiencies. All known microbody enzymes that are synthesized in a form similar in size to the mature molecule, except catalase, contain one of these tripeptide sequences at their carboxyl terminus.
- 日本植物生理学会の論文
著者
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NISHIMURA Mikio
Department of Cell Biology, National Institute for Basic Biology
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HAYASHI Makoto
Department of Basic Medical Research and Education, Ehime University Graduate School of Medicine
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Moriya Kei
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Hayashi M
Department Of Cell Biology National Institute For Basic Biology
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Nishimura Mikio
Department Of Cell Biology National Institute For Basic Biology
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Nishimura Mikio
Dept. Cell Biol. Nat. Inst. Basic. Biol.
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KONDO Maki
Department of Cell Biology, National Institute for Basic Biology
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Kojima M
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Aoki M
Hitachi Ltd. Central Research Laboratory
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Kojima Michiyuki
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Kondo Maki
Department Of Cell Biology National Institute For Basic Biology
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Aoki Masahiro
Department of Cell Biology, National Institute for Basic Biology
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Aoki Masahiro
Hitachi Ltd. Central Research Laboratory
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Aoki M
Univ. Tsukuba Shizuoka Jpn
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Nishimura Mikio
National Inst. For Basic Biology Okazaki Jpn
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Komai Michio
Nof Corporation
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Hayashi Makoto
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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