Expression and Localization of a 36-kDa Peptide Derived from a 24-kDa Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Sweet Potato Cells in Suspension Culture

スポンサーリンク

概要

• 論文の詳細を見る

• Expression and localization of a 36-kDa peptide (VP36) derived from a 24-kDa vacuolar protein (VP24) precursor were investigated in anthocyanin-producing sweet potato cells (Ipomoea batatas). VP24 is one of the major proteins in the anthocyanin-containing vacuoles, and synthesized as a large precursor protein that contains a C-terminal region in addition to the mature domain [Xu et al., Plant Physiol. (2001) 125: 447]. The accumulation patterns of both VP36 and VP24 were closely correlated with the accumulation of anthocyanin in the vacuoles. The immunocytochemical analysis using antibodies against the fusion protein containing a portion of the C-terminal peptide showed that VP36 was localized in intravacuolar pigmented globules (cyanoplasts) in a manner similar to VP24. These results further suggest that VP36 is a peptide derived from the VP24 protein precursor. Both VP36 and VP24 peptides are probably involved in formation of cyanoplasts in the anthocyanin-producing sweet potato cells.

• 日本植物細胞分子生物学会の論文
• 2001-09-01

著者

• Nogawa Masahiro

  Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• NISHIMURA Mikio

  Department of Cell Biology, National Institute for Basic Biology

• Yamada Kenji

  Department of Surgery, Nagasaki medical center of neurology

• Moriya Kei

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Yamada Kenji

  Department Of Cell Biology National Institute For Basic Biology

• Nishimura M

  Dep. Of Cell Biology National Inst. For Basic Biology

• Nishimura Mikio

  Department Of Cell Biology National Institute For Basic Biology

• Nishimura Mikio

  Dept. Cell Biol. Nat. Inst. Basic. Biol.

• KONDO Maki

  Department of Cell Biology, National Institute for Basic Biology

• Kojima M

  Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine

• XU Wenxin

  Graduate School of Science and Technology, Shinshu University

• SHIOIRI Hidenari

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• KOJIMA Mineo

  Graduate School of Science and Technology, Shinshu University

• NOZUE Masayuki

  Graduate School of Science and Technology, Shinshu University

• Kojima Michiyuki

  Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine

• Kojima Mineo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kondo Maki

  Department Of Cell Biology National Institute For Basic Biology

• Kondo Maki

  Dept. Cell Biol. Nat. Inst. Basic Biol.

• Nishimura Mikio

  Dep. Of Cell Biology National Inst. For Basic Biology

• Xu Wenxin

  Graduate School Of Science And Technology Shinshu University

• Kojima M

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Nogawa Masahiro

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Nogawa Masahiro

  Department Of Bioengineering Nagaoka University Of Technology

• Nishimura Mikio

  National Inst. For Basic Biology Okazaki Jpn

• Shioiri Hidenari

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Komai Michio

  Nof Corporation

• Yamada Kenji

  Department Of Biopharmaceutics Meiji College Of Pharmacy:department Of Radiology:division Of Clinica

• Yamada Kenji

  Department Of Biochemistry Kanazawa Medical University

• YAMADA Kenji

  Department of Applied Chemistry, Faculty of Engineering, Chiba University

関連論文

• Analysis of a change in bacterial community in different environments with addition of chitin or chitosan(ENVIRONMENTAL BIOTECHNOLOGY)
• Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
• Development of Simple and Efficient in Planta Transformation Method for Rice (Oryza sativa L.) Using Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
• Cytosolic Aconitase Participates in the Glyoxylate Cycle in Etiolated Pumpkin Cotyledons : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
• Spatial Patterns of Sucrose-Inducible and Polygalacturonic Acid-Inducible Expression of Genes That Encode Sporamin and β-Amylase in Sweet Potato : GENE STRUCTURE AND EXPRESSION
• Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display
• Molecular Cloning of a Defense-Response-Related Cytochrome P450 Gene from Tobacco
• The Involvement of Tonoplast Proton Pumps and Na^+(K^+)/H^+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
• A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
• A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
• β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
• Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells
• Detection and Characterization of a Vacuolar Protein(VP24) in Anthocyanin Producing Cells of Sweet Potato in Suspension Culture : PROTEINS, ENZYMES AND METABOLISM : STRUCTURE AND FUNCTION OF CELLS
• AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells
• An ER-Localized Form of PV72, a Seed-Specific Vacuolar Sorting Receptor, Interferes the Transport of an NPIR-Containing Proteinase in Arabidopsis Leaves
• Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana
• The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis
• A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
• Proteomic Analysis of Leaf Peroxisomal proteins in Greening Cotyledons of Arabidopsis thaliana
• Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana
• Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP : Dynamic Morphology and Actin-Dependent Movement
• Activation of Arabidopsis Vacuolar Processing Enzyme by Self-Catalytic Removal of an Auto-Inhibitory Domain of the C-Terminal Propeptide
• Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid β-Oxidation
• Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons
• Pumpkin Peroxisomal Ascorbate Peroxidase is Localized on Peroxisomal Membranes and Unknown Membranous Structures
• THE ANALYSIS OF THE TRANSPORT PATHWAY TO VACUOLES DIRECTLY FROM ER
• BINDING ANALYSIS OF A VACUOLAR SORTING RECEPTOR, PV72
• A NOVEL VACUOLAR MEMBRANE PROTEIN, MP73,IS TRANSPORTED TO VACUOLES VIA PAC VESICLES
• THE PROCESSING MECHANISM AND THE PHYSIOLOGICAL FUNCTION OF VEGETATIVE TYPE VACUOLAR PROCESSING ENZYME IN HIGHER PLANTS
• Distribution and characterization of peroxisomes in Arabidopsis thaliana by visualization with GFP
• Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana
• Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
• Expression and Localization of a 36-kDa Peptide Derived from a 24-kDa Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Sweet Potato Cells in Suspension Culture
• Tissue and Intracellular Localization of Indican and the Purification and Characterization of Indican Synthase from Indigo Plants
• A Novel Isoenzyme of Ascorbate Peroxidase Localized on Glyoxysomal and Leaf Peroxisomal Membranes in Pumpkin : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
• Development of Microbody Membrane Proteins during the Transformation of Glyoxysomes to Leaf Peroxisomes in Pumpkin Cotyledons : MEMBRANES AND BIOENERGETICS : STRUCTURE AND FUNCTION OF CELLS
• Cloning and Sequencing of cDNA for Glycolate Oxidase from Pumpkin Cotyledons and Northern Blot Analysis
• Characterization of Intravacuolar Pigmented Structures in Anthocyanin-Containing Cells of Sweet Potato Suspension Cultures
• The Sequence and Expression of the γ-VPE Gene, One Member of a Family of Three Genes for Vacuolar Processing Enzymes in Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
• ISOLATION AND CHARACTERIZATION OF ARABIDOPSIS MUTANTS THAT HAVE DEFECTS IN THE PROCESSING OF SEED STORAGE PROTEINS
• THE LOCALIZATION OF PUMPKIN PV72,A SORTING RECEPTOR FOR VACUOLAR PROTEINS
• Characterization of a putative vacuolar sorting receptor, PV72,in developing pumpkin seeds
• TRANSPORT PATHWAYS FOR SEED STORAGE PROTEINS TO THE VACUOLES OF HIGHER PLANTS
• ANALYSIS OF THE VACUOLAR TARGETING SIGNAL OF PUMPKIN PRO2S ALBUMIN
• MOLECULAR CHARACTERIZATION OF ARABIDOPSIS CO-CHAPERONINS.
• MULTIPLE FUNCTIONAL PROTEINS ARE PRODUCED BY CLEAVING ASN-GLN BONDS OF A SINGLE PRECURSOR BY VACUOLAR PROCESSING ENZYME
• A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor
• A Rapid Increase in the Level of Binding Protein (BiP) is Accompanied bySynthesis and Degradation of Storage Proteins in Pumpkin Cotyledons
• A NOVEL MEMBRANE PROTEIN, PMP38,IS LOCALIZED ON PLANT GLYOXYSOMAL MEMBRANES
• INTRACELLULAR TRANSPORT OF A LEAF PEROXISOMAL 27-kDa PROTEIN FROM Arabidopsis thaliana
• Microbody Defective Mutants of Arabidopsis
• MOLECULAR MECHANISM FOR TARGETING OF PLANT CATALASE TO MICROBODIES
• Hydroxypyruvate Reductase with a Carboxy-Terminal Targeting Signal to Microbodies is Expressed in Arabidopsis
• cDNA Cloning and Expression of a Gene for Isocitrate Lyase in Pumpkin Cotyledons
• Functional Transformation of Microbodies in Higher Plant Cells
• Buckwheat Transformed with cDNA of a Rice MADS Box Gene Is Stimulated in Branching
• Expression of Two Trichoderma reesei Xylanases in the Fission Yeast Schizosaccharomyces pombe
• Xylanase Induction by L-Sorbose in a Fungus, Trichoderma reesei PC-3-7
• Functional Classification of Arabidopsis Peroxisome Biogenesis Factors Proposed from Analyses of Knockdown Mutants
• Oligomeric Proteins Containing N-Terminal Targeting Signals are Imported into Peroxisomes in Transgenic Arabidopsis
• Accumulation of a Fusion Protein Containing 2S Albumin Induces Novel Vesicles in Vegetative Cells of Arabidopsis
• ASCORBATE PEROXIDASE THAT IS LOCALIZED IN MICROBODY MEMBRANES
• CHARACTERIZATION OF VEGETATIVE-TYPE VACUOLAR PROCESSING ENZYME of Arabidopsis
• ARABIDOPSIS ped2 MUTANT ACCUMULATES PRECURSOR WITH HIGH MOLECULAR MASS FOR PTS2-TYPE PEROXISOMAL PROTEINS
• PURIFICATION AND CHARACTERIZATION OF A PEROXISOMAL SHORT-CHAIN ACYL-COA OXIDASE FROM PUMPKIN
• MOLECULAR CHARACTERIZATION OF ACYL-CoA OXIDASE IN PUMPKIN
• ARABIDOPSIS ped1 MUTANT THAT HAS DEFECT IN THIOLASE GENE
• Glyoxysomal Malate Dehydrogenase in Pumpkin : Cloning of a cDNA and Functional Analysis of Its Presequence
• Changes in Targeting Efficiencies of Proteins to Plant Microbodies Caused by Amino Acid Substitutions in the Carboxy-terminal Tripeptide
• Cryptochromes and Phytochromes Synergistically Regulate Arabidopsis Root Greening under Blue Light
• Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation
• Behavior of Vacuoles during Microspore and Pollen Development in Arabidopsis thaliana
• Development of Enzymes of the Glyoxylate Cycle during Senescence of Pumpkin Cotyledons
• Glyoxylate Cycle Enzymes in Peroxisomes Isolated from Petals of Pumpkin (Cucurbita sp.) during Senescence
• Cloning and analysis of genes coding for chitinolytic enzymes from the novel chitinolytic bacterium Chitiniphilus shinanonensis
• Role of Cellulose-Binding Domain of Exocellulase I from White Rot Basidiomycete Irpex lacteus
• Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660
• The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases
• Arabidopsis MUTANTS THAT HAVE DEFECTS ON GLYOXYSOMAL FUNCTIONS
• A Vacuolar Processing Enzyme in Maturing and Germinating Seeds : Its Distribution and Associated Changes during Development
• CHARACTERIZATION OF A PROTEIN COMPONENT, DV100, OF THE DENSE VESICLES FROM MATURING PUMPKIN SEEDS
• Small GTP-Binding Proteins are Associated with the Vesicles That are Targeted to Vacuoles in Developing Pumpkin Cotyledons : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
• Ectopic Expression of an Esterase, Which is a Candidate for the Unidentified Plant Cutinase, Causes Cuticular Defects in Arabidopsis thaliana
• Development of Simple and Efficient in Planta Transformation Method for Wheat (Triticum aestivum L.) Using Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
• In Planta Transformation of Kenaf Plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
• In Planta Transformation of Mulberry Trees (Morus alba L.) by Agrobacterium tumefaciens
• Isolation and Characterization of a New Virulence Gene (abvA) of Agrobacterium tumefaciens
• EXPRESSION AND ACTIVATION OF POLYPHENOL OXIDASE ISOFORMS IN SWEET POTATO CELLS IN SUSPENSION CULTURE
• M-31 Mutant (virA::Tn5) of Agrobacterium tumefaciens Is Capable of Transferring Its T-DNA into the Nucleus of Host Cell, but Incapable of Integrating It into the Chromosome
• Development of a Simple and Efficient Method for Transformation of Buckwheat Plants (Fagopyrum esculentum) Using Agrobacterium tumefaciens
• PRIMARY STRUCTURE AND FUNCTIONAL ANALYSIS OF 24-KDA VACUOLAR PROTEIN IN ANTHOCYANIN-PRODUCING SWEET POTATO CELLS IN SUSPENSION CULTURE
• CHRACTERIZATION OF POLYPHENOL OXIDASE-PROCESSING ENZYME IN SWEET POTATO CELLS IN SUSPENSION CULTURE
• ACTIVATION AND MONOPHENOL HYDROXYLASE ACTIVITY OF POLYPHENOL OXIDASE IN SWEET POTATO SUSPENSION CULTURES
• Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
• Cloning and Characterization of a Chitosanase Gene from the Plant Pathogenic Fungus Fusarium solani
• Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae
• Chitosanase from the Plant Pathogenic Fungus, Fusarium solani f. sp. phaseoli : Purification and Some Properties
• Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis
• Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria

もっと見る 閉じる

スポンサーリンク