Chitosanase from the Plant Pathogenic Fungus, Fusarium solani f. sp. phaseoli : Purification and Some Properties
スポンサーリンク
概要
- 論文の詳細を見る
Among 162 strains of the genus Fusarium tested, 22 strains, mainly from F. solani and F. splendens, formed halos on chitosan-containing agar medium. Chitosanase secreted in the culture by the most effective producer, F. solani f. sp. phaseoli SUF386, was further investigated. N-Acetylglucosamine (GlcNAc) used as a carbon source was most effective for production of chitosanase. Chitosan, a substrate for chitosanase, inhibited cell growth completely and abolished production of chitosanase when used as a carbon source in the liquid medium. Chitosanase purified from the culture filtrate had a molecular mass of 36kDa, and showed a maximum activity at pH 5.6 and 40℃. The enzyme catalyzed the hydrolysis of chitopentaose, chitosan (70% and 100% deacetylation), and glycolchitosan, but showed little activity toward chitobiose, chitotriose, chitotetraose, glycol chitin, and carboxymethyl cellulose. A rapid reduction in the viscosity of chitosan solutions suggested that the enzyme catalyzed an endo-type cleavage reaction.
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
-
Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
-
Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
-
Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
-
Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Ohno Yasuyo
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
関連論文
- Analysis of a change in bacterial community in different environments with addition of chitin or chitosan(ENVIRONMENTAL BIOTECHNOLOGY)
- 第4回(平成19年度)日本学術振興会賞受賞 : 押川正毅氏(学界ニュース)
- 24pTL-8 スピン1/2XXZ鎖のストリング相関関数の漸近挙動とその応用(量子スピン系,領域11,統計力学,物性基礎論,応用数学,力学,流体物理)
- 18aWA-2 1次元ハバード模型の高次の保存量のハーフフィリングにおける強結合展開(量子スピン系,領域11,原子・分子,量子エレクトロニクス,放射線物理)
- 18aWA-3 Delta=1/2 XXZ鎖のエンタングルメントエントロピー(量子スピン系,領域11,原子・分子,量子エレクトロニクス,放射線物理)
- 25aXD-3 スピン1/2ハイゼンベルクXXZ鎖のストリング相関関数(25aXD 量子スピン系,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 29pUA-2 ハイゼンベルクスピン鎖の基底状態相関関数(29pUA 領域11シンポジウム:可積分量子スピン系の新展開:相関関数と有限温度の厳密解,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 21pXL-6 一次元ハイゼンベルクXXX模型の基底状態の相関関数の厳密計算(量子スピン系,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 19aPS-76 一次元Heisenberg模型の有限温度相関函数の高温展開(領域11ポスターセッション,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- Heisenbergスピン鎖の相関関数(最近の研究から)
- 相関関数とゼータ関数 (特集 可解格子模型入門)
- Evaluation of Dynamic Spin Structure Factor for the Spin-1/2 XXZ Chain in a Magnetic Field(General)
- 12aTK-2 XXX 鎖の相関関数の計算(量子可積分系, 領域 11)
- Next Nearest-Neighbor Correlation Functions of the Spin-1/2 XXZ Chain at Massive Region(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- 31aPS-9 スピン-1/2 XXZ 鎖の Emptiness Formation Probability の漸近的ふるまい
- 28aWE-9 Δ=1/√XXZ 鎖の相関関数
- 量子スピン鎖における新しい相関関数-emptiness formation probability-に関する最近の発展
- 18aRC-4 1次元等方的XY模型のEmptiness Formation Probability
- 18aRC-3 熱力学ベーテ仮説と量子転送行列の等価性
- Development of Simple and Efficient in Planta Transformation Method for Rice (Oryza sativa L.) Using Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
- Expression and Localization of a 36-kDa Peptide Derived from a 24-kDa Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Sweet Potato Cells in Suspension Culture
- Analysis of a Promoter-Like Region in Flavobacterium Which Controls 6-Aminohexanoic Acid Linear Oligomer Hydrolase Gene Expression in Escherichia coli
- Involvement of Parathyroid Hormone-Related Peptide in Cell Proliferation Activity of Human Uterine Leiomyomas
- Fermionic R-Operator and Integrability of the One-Dimensional Hubbard Model
- Isolation of Sake Yeast Strains Possessing Various Levels of Succinate- and/or Malate-Producing Abilities by Gene Disruption or Mutation
- Effect of Gene Disruptions of the TCA Cycle on Production of Succinic Acid in Saccharomyces cerevisiae
- Buckwheat Transformed with cDNA of a Rice MADS Box Gene Is Stimulated in Branching
- Expression of Two Trichoderma reesei Xylanases in the Fission Yeast Schizosaccharomyces pombe
- Xylanase Induction by L-Sorbose in a Fungus, Trichoderma reesei PC-3-7
- Effect of Salt Stress on Plasma Membrane Permeability and Lipid Saturation in the Salt-Tolerant Yeast Zygosaccharomyces rouxii
- Isolation and Characterization of Zygosaccharomyces rouxii Mutants Defective in Proton Pumpout Activity and Salt Tolerance
- Expression of Genes Encoding Membrane-bound Hydrogenase in Pseudomonas hydrogenovora under Autotrophic Condition Is Dependent on Two Different Promoters
- Cloning of the SAT1 Gene Concerned with Salt Tolerance of the Yeast Zygosaccharomyces rouxii
- Gene Cloning of an Endoglucanase from the Basidiomycete Irpex lacteus and Its cDNA Expression in Saccharomyces cerevisiae
- Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Cloning and analysis of genes coding for chitinolytic enzymes from the novel chitinolytic bacterium Chitiniphilus shinanonensis
- Analysis of Essential Carboxylic Amino Acid Residues for Catalytic Activity of Fungal Chitosanases by Site-Directed Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification, Cloning, and Sequence Analysis of β-N-Acetylglucosaminidase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Effect of Administration with the Extract of Gymnema sylvestre R. Br Leaves on Lipid Metabolism in Rats
- Effect of Long Term-Administration with Gymnema sylvestre R.B_R on Plasma and Liver Lipid in Rats
- Role of Cellulose-Binding Domain of Exocellulase I from White Rot Basidiomycete Irpex lacteus
- Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660
- The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases
- 再録 Isolation of sake yeast mutants producting a higher level of ethyl caproate and/or iso-amyl acetate
- Isolation of Sake Yeast Mutants Producing a High Level of Ethyl Caproate and/or Isoamyl Acetate
- Purification and Characterization of Chitosanase and Exo-β-D-Glucosaminidase from a Koji Mold, Aspergillus oryzae IAM2660
- Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus
- Isolation and Transcriptional Analysis of a Cellulase Gene(cel1) from the Basidiomycete Irpex lacteus
- Action of Exo- and Endo-Type Cellulases from Irpex lacteus on Valonia Cellulose
- A Transient Increase of Phenylalanine Ammonia-lyase Transcript in Kinetin-treated Tobacco Callus
- Improvement of a Fixed Bed Bioreactor for Plant Cell Culture
- Development of Simple and Efficient in Planta Transformation Method for Wheat (Triticum aestivum L.) Using Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
- In Planta Transformation of Kenaf Plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens(PLANT BIOTECHNOLOGY)
- In Planta Transformation of Mulberry Trees (Morus alba L.) by Agrobacterium tumefaciens
- Isolation and Characterization of a New Virulence Gene (abvA) of Agrobacterium tumefaciens
- EXPRESSION AND ACTIVATION OF POLYPHENOL OXIDASE ISOFORMS IN SWEET POTATO CELLS IN SUSPENSION CULTURE
- M-31 Mutant (virA::Tn5) of Agrobacterium tumefaciens Is Capable of Transferring Its T-DNA into the Nucleus of Host Cell, but Incapable of Integrating It into the Chromosome
- Race 3, a new race of Fusarium oxysporum f. sp. lactucae determined by a differential system with commercial cultivars
- Development of a Simple and Efficient Method for Transformation of Buckwheat Plants (Fagopyrum esculentum) Using Agrobacterium tumefaciens
- PRIMARY STRUCTURE AND FUNCTIONAL ANALYSIS OF 24-KDA VACUOLAR PROTEIN IN ANTHOCYANIN-PRODUCING SWEET POTATO CELLS IN SUSPENSION CULTURE
- CHRACTERIZATION OF POLYPHENOL OXIDASE-PROCESSING ENZYME IN SWEET POTATO CELLS IN SUSPENSION CULTURE
- ACTIVATION AND MONOPHENOL HYDROXYLASE ACTIVITY OF POLYPHENOL OXIDASE IN SWEET POTATO SUSPENSION CULTURES
- Phylogenetic relationships between the lettuce root rot pathogen Fusarium oxysporum f. sp. lactucae races 1,2, and 3 based on the sequence of the intergenic spacer region of its ribosomal DNA
- Inactivation of Hexokinase and Glucose-6-phosphate Dehydrogenase during Immobilization by Polyacrylamid Gel Entrapping
- Hydrogen evolution by a thermophilic blue-green alga Mastigocladus laminosus
- Mixed Culture of Mycotorula japonia and Pseudomonas oleovorans on Two Hydrocarbons
- Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Gene Cloning of Cellobiohydrolase II from the White Rot Fungus Irpex lacteus MC-2 and Its Expression in Pichia pastoris
- Gene Cloning, Expression, and Characterization of a Second β-N-Acetylglucosaminidase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Effect of gene disruptions of the TCA cycle on production of succinic acid in Saccharomyces cerevisiae
- COUMARIN UP-TAKE SYSTEM IN TOBACCO CULTURED CELL
- INDUCTION AND GENETIC ANALYSIS OF PHENYLALANINE AMMONIA-LYASE IN TOBACCO COULTURED CELL
- Effects of Kinetin on _L-Phenylalanine Ammonia-lyase Activity in Tobacco Cell Culture(Biological Chemistry)
- Cloning and Characterization of a Chitosanase Gene from the Plant Pathogenic Fungus Fusarium solani
- Expression Pattern and Gene Structure of Phenylalanine Ammonia-Lyase in Pharbitis nil
- Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae
- Chitosanase from the Plant Pathogenic Fungus, Fusarium solani f. sp. phaseoli : Purification and Some Properties
- Purification and Some Properties of 2, 4-D-Activated Scooletin Glucosyltransferase from Tobacco Cells
- Transcription of a Gene for 6-Aminohexanoic Acid Linear Oligomer Hydrolase of Flavobacterium sp. KI72 in Escherichia coli
- Cellulose and Xylan Degrading Enzymes of the Plant Pathogenic Fungus, Fusarium oxyspomm SUF850(Microbiology & Fermentation Industry)
- COLLECTION AND ANALYSIS OF ARABIDOPSIS COLOR MUTANTS
- Analysis of transposon Tag1 related elements
- Kinetic Studies on Enzyme Production by Microbes : (I) On Kinetic Models
- HETEROLOGOUS EXPRESSION AND PROPERTIES OF GLUCOSYLTRANSFERASE NTGT1 FROM TOBACCO
- THE EFFECT OF METHYL JASMONATE ON 2,4-D STIMULATED SCOPOLETIN UPTAKE BY TOBACCO CELLS
- 325 Regulation of Phenylalanine Ammonia-lyase in Tobacco Cell Culture
- Preferential Synthesis of Extracellular Glucoamylase by Aspergillus niger : Stability of mRNA Associated with Membrane-boud Polysomes in Nongrowing Mycelia
- Kinetic Studies on Enzyme Production by Mictobes : (III) Process Kinetics of Glucamylase Production by Aspergillus niger
- Cell Aggregation and Elongated Cell Morphology Caused by a Mutation Conferring Increased Sensitivity to Indole-3-Acetic Acid in the Yeast : Saccharomyces cerevisiae
- Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis
- Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
- Growth Kinetics of Hydrogen Bacterium Alcaligenes hydrogenophilus
- Effects of Plant Hormones on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures
- Effects of Kinetin on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures
- Effects of Nutritional Factors on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures
- The acid-catalyzed rearrangement of 1-allylindoles. A hypothesis for the biogenesis of echinulin-type compounds.