Purification and Characterization of Chitosanase and Exo-β-D-Glucosaminidase from a Koji Mold, Aspergillus oryzae IAM2660
スポンサーリンク
概要
- 論文の詳細を見る
Chitosan-degrading activity was detected in the culture fluid of Aspergillus oryzae, A. sojae, and A. flavus among various fungal strains belonging to the genus Aspergillus. One of the strong producers, A. oryzae IAM2660 had a higher level of chitosanolytic activity when N-acetylglucosamine (GlcNAc) was used as a carbon source. Two chitosanolytic enzymes, 40 kDa and 135kDa in molecular masses, were purified from the culture fluid of A. oryzae IAM2660. Viscosimetric assay and an analysis of reaction products by thin-layer chromatography clearly indicated the endo-and exo-type cleavage manner for the 40-kDa and 135-kDa enzymes, respectively. The 40-kDa enzyme, designated chitosanase, catalyzed a hydrolysis of glucosamine (GlcN) oligomers larger than pentamer, glycol chitosan, and chitosan with a low degree of acetylation (0-30%). The 135-kDa enzyme, named exo-β-D-glucosaminidase, released a single GlcN residue from the GlcN oligomers and chitosan, but did not release GlcNAc residues from either GlcNAc oligomer or colloidal chitin.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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SHIMOSAKA MAKOTO
Department of Applied Biology, Shinshu University
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Zhang Xiaoyong
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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OKAZAKI MITSUO
Gene Research Center, Shinshu University, and Department of Applied Biology, Faculty of Textile Scie
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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KODAIRA Ritsuko
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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ZHANG Xiao-Yong
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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DAI An-Lan
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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KUROIWA Kouji
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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DAI AnLan
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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ZHANG XueKun
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Dai Anlan
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kuroiwa Kouji
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
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城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kodaira Ritsuko
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Zhang Xue-Kun
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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OKAZAKI Mitsuo
Gene Research Center, Shinshu University
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