Gene Cloning of an Endoglucanase from the Basidiomycete Irpex lacteus and Its cDNA Expression in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
A gene (cen1) coding for an endoglucanase I (En-1) was isolated from white rot fungus Irpex lacteus strain MC-2. The cen1 ORF was comprised of 399 amino acid residues and interrupted by 14 introns. The deduced amino acid sequence of the cen1 ORF revealed a multi-domain structure composed of a cellulose-binding domain, a Ser-/Thr-rich linker, and a catalytic domain from the N-terminus. It showed a significant similarity to those of other endoglucanases that belong to family 5 of glycosyl hydrolases. cen1 cDNA was inserted into a yeast expression vector, YEpFLAG-1, and introduced into Saccharomyces cerevesiae. The resulting S. cerevisiae transformant secreted a recombinant En-1 that had enzymatic properties similar to the original En-1. A strong synergistic effect for a degradation of Avicel and phosphoric acid swollen cellulose was observed when recombinant En-1 was used together with a major exo-type cellobiohydrolase I of I. lacteus MC-2.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
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ODA MAKOTO
Department of General and Cardiothoracic Surgery, Kanazawa University School of Medicine
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Toda Hiroshi
Department Of Surgery Seirei Sumiyoshi Hospital
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TAKADA SATOSHI
Department of Urology, Nara Medical University
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OKAZAKI MITSUO
Department of Applied Biology, Shinshu University
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SHIMOSAKA MAKOTO
Department of Applied Biology, Shinshu University
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Shiroishi Masahiro
Agricultural Technology Institute Of Nagano Farmers' Federation
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Shiroishi Masahiro
Department Of Physics Graduate School Science University Of Tokyo
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Shiroishi Masahiro
Department Of Physics Graduate School Of Science University Of Tokyo
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Oda Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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KANDA Takahisa
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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AMANO Yoshihiko
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University
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Kanda Takahisa
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
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Amano Yoshihiko
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Takada Satoshi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Toda Hiroshi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Toda Hiroshi
Department Of Anesthesia Kyoto University Hospital
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Arikawa Y
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
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Kuroyanagi Tomoko
Faculty Of Textile Science And Technology Shinshu University
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