Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
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概要
- 論文の詳細を見る
A stable bacterial community expressing strong chitinolytic activity was constructed by mixing and cultivating chitinolytic bacteria collected from different natural sources. The DNA fragment pattern, after PCR-denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes using total DNA prepared from whole cells, indicated that the community was composed of four dominant bacterial species. All four species were isolated on agar medium, and one strain, SAY3, was deduced to be a novel species belonging to a new genus based on the 16S rRNA nucleotide sequence. The other strains showed high similarity in their 16S rRNA sequences to those of previously identified bacteria (Acinetobacter and Microbacterium). Strain SAY3 degraded and utilized larger particles of chitin faster than the community, indicating that it plays an important role in the chitin degradation conferred by the community.
- 社団法人 日本農芸化学会の論文
- 2010-03-23
著者
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Sato Kazuaki
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi Goro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Shimosaka Makoto
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi G
信州大・繊維・応生系
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Sato Kazuaki
信州大・繊維・応生系
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Nogawa Masahiro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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KATO Yuichi
信州大・繊維・応生系
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KATO Yuichi
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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FUKAMACHI Ayabi
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi Goro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Shimosaka Makoto
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Fukamachi Ayabi
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kato Yuichi
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sato Kazuaki
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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