Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Chitiniphilus shinanonensis type strain SAY3^T is a strongly chitinolytic bacterium, originally isolated from the moat water in Ueda, Japan. To elucidate the chitinolytic activity of this strain, 15 genes (chiA-chiO) coding for putative chitin-degrading enzymes were isolated from a genomic library. Sequence analysis revealed the genes comprised 12 family 18 chitinases, a family 19 chitinase, a family 20 β-N-acetylglucosaminidase, and a polypeptide with a chitin-binding domain but devoid of a catalytic domain. Two operons were detected among the sequences: chiCDEFG and chiLM. The gene coding for the polypeptide (chiN) showed sequence similarity to family 19 chitinases and was successfully expressed in Escherichia coil. ChiN demonstrated a multi-domain structure, composed of the N-terminal, two chitin-binding domains connected by a Pro- and Thr-rich linker, and a family 19 catalytic domain located at the C-terminus. The recombinant protein rChiN catalyzed an endotype cleavage of N-acetyl-D-glucosamine oligomers, and also degraded insoluble chitin and soluble chitosan (degree of deacetylation of 80%). rChiN exhibited an inhibitory effect on hyphal growth of the fungus Trichoderma reesei. The chitinbinding domains of ChiN likely play an important role in the degradation of insoluble chitin, and are responsible for a growth inhibitory effect on fungi.
- 公益社団法人日本生物工学会の論文
著者
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Sato Kazuaki
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Shiroishi Masahiro
Agricultural Technology Institute Of Nagano Farmers' Federation
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Shiroishi Masahiro
Department Of Physics Graduate School Science University Of Tokyo
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Shiroishi Masahiro
Department Of Physics Graduate School Of Science University Of Tokyo
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Taguchi G
信州大・繊維・応生系
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Sato Kazuaki
信州大・繊維・応生系
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Nogawa Masahiro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
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HUANG Lanxiang
信州大・繊維・応生系
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KATO Yuichi
信州大・繊維・応生系
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城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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KATO Yuichi
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi Goro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Shimosaka Makoto
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Huang Lanxiang
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Garbulewska Ewelina
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Huang Lanxiang
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Garbulewska Ewelina
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kato Yuichi
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sato Kazuaki
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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