Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes
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概要
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Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.
- 2010-11-23
著者
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INATOMI Satoshi
Mushroom Research Laboratory, Hokuto Corporation
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Shimosaka Makoto
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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OKUHARA Toru
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Okamoto Tomomitsu
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Taguchi Goro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamada Masato
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okuhara Toru
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiya Shuichi
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Inatomi Satoshi
Mushroom Research Laboratory Hokuto Corporation
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Inatomi Satoshi
Mushroom Laboratory Hokuto Corporation
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Shimosaka Makoto
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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