Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes.
- 公益社団法人日本生物工学会の論文
- 2013-04-00
著者
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Yamada Masato
Department Of Biotechnology Toyama Prefectural University
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SEKIYA Shuichi
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi Goro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okuhara Toru
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiya Shuichi
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Inatomi Satoshi
Mushroom Laboratory Hokuto Corporation
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Shimosaka Makoto
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Shibata Kou
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Yoshida Masumi
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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