Construction of a Practical SCAR Marker Linked to Clubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes
スポンサーリンク
概要
- 論文の詳細を見る
A restriction fragment length polymorphism (RFLP) marker, HC352b, has identified as closely linked to the CRa locus responsible for clubroot resistance (CR) in a study dissecting CR loci in Chinese cabbage (Brassica rapa L. ssp pekinensis). Unfortunately, the RFLP pattern, including HC352b detected by the cDNA clone HC352, was complicated and confused in its interpretation because it represented multiple-copy loci. To provide a practical HC352b marker for CR breeding programs, a sequence characterized amplified region (SCAR) marker was constructed along the analysis of HC352 genes, followed by its evaluation for CR selection. Schematic characterization of signals was achieved by identification of HC352 homologous genes with their restriction sites, employing CRa-positive and -negative doubled haploid (DH) lines. Genomic sequence information from a set of HC352 homologous genes was analyzed to identify the CRa linked paralog HC352b, followed by the successful designation of a SCAR marker. This correctly predicted the CR phenotypes of all tested individuals of F2 and back-crossed progenies.
- 園芸学会の論文
著者
-
Taguchi Goro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
-
Matsumoto Etsuo
Nagano Vegetable And Ornamental Crops Experiment Station Nagano
-
Matsumoto Etsuo
Nagano Vegetable & Ornamental Crops Experiment Station
-
Hayashida Nobuaki
Division of Gene Research, Shinshu University
-
Takabatake Yuri
Division of Gene Research, Shinshu University
-
Nakazawa Norihiro
Division of Gene Research, Shinshu University
-
Aruga Daisuke
Division of Gene Research, Shinshu University
-
Nakanishi Hiromitsu
Division of Gene Research, Shinshu University
-
Sakamoto Koji
Takii Plant Breeding & Experiment Station
-
Hayashida Nobuaki
Division Of Gene Research Shinshu University
-
Taguchi G
信州大・繊維・応生系
-
Aruga Daisuke
Division Of Gene Research Shinshu University
-
Nakanishi H
Division Of Oncological Pathology Aichi Cancer Center Research Institute
-
Taguchi Goro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Nakazawa Norihiro
Division Of Gene Research Shinshu University
-
Hayashida Nobuaki
Division Of Gene Research Department Of Life Science Research Center For Human And Environmental Sci
-
Nakanishi Hiromitsu
Division Of Gene Research Shinshu University
-
Sakamoto Koji
Takii Plant Breeding & Experiment Station
-
Takabatake Yuri
Division Of Gene Research Shinshu University
-
Taguchi G
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Aruga D
Division of Gene Research, Shinshu University
-
Sakamoto K
Takii Plant Breeding & Experiment Station
-
Matsumoto E
Nagano Vegetable & Ornamental Crops Experiment Station
-
Hayashida Nobuaki
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
関連論文
- Analysis of a change in bacterial community in different environments with addition of chitin or chitosan(ENVIRONMENTAL BIOTECHNOLOGY)
- New animal model of glandular stomach carcinogenesis in Mongolian gerbils infected with Helicobacter pylori and treated with a chemical carcinogen
- N-Methyl-N-nitrosourea Concentration-dependent, Rather than Total Intake-dependent, Induction of Adenocarcinomas in the Glandular Stomach of BALB/c Mice
- Dose-dependent Promoting Effects of Catechol on Glandular Stomach Carcinogenesis in BALB/c Mice Initiated with N-Methyl-N-nitrosourea
- Rare Occurrence of ras and p53 Gene Mutations in Mouse Stomach Tumors Induced by N-Methyl-N-nitrosourea
- A polymorphism of C-to-T substitution at -31 IL1B is associated with the risk of advanced gastric adenocarcinoma in a Japanese population
- Gastric and intestinal mixed and solely intestinal types of intestinal metaplasia in the human stomach
- Dose-dependent Induction of Both Pepsinogen-altered Pyloric Glands and Adenocarcinomas in the Glandular Stomach of C3H Mice Treated with N-Methyl-N-nitrosourea
- Growth Characteristics in the Initial Stage of Micrometastasis Formation by Bacterial LacZ Gene-tagged Rat Prostatic Adenocarcinoma Cells
- Development of Aberrant Crypt Foci Involves a Fission Mechanism as Revealed by Isolation of Aberrant Crypts
- Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet-cell differentiation from liver metastasis
- Establishment and Characterization of Two Cell Lines from N-Methyl-N-nitrosourea-induced Mouse Glandular Stomach Carcinomas
- Targeted delivery of oligomannose-coated liposome to the omental micrometastasis by peritoneal macrophages from patients with gastric cancer
- Establishment and Characterization of Three Androgen-independent, Metastatic Carcinoma Cell Lines from 3,2'-Dimethyl-4-aminobiphenyl-induced Prostatic Tumors in F344 Rats
- Detection of p16 promoter hypermethylation in serum of gastric cancer patients
- Chemosensitivity of peritoneal micrometastases as evaluated using a green fluorescence protein (GFP)-tagged human gastric cancer cell line
- Detection of Tumor DNA in Serum of Colorectal Cancer Patients
- Colorectal Cancers with both p16 and p14 Methylation Show Invasive Characteristics
- Detection of Carcinoembryonic Antigen-expressing Free Tumor Cells in Peritoneal Washes from Patients with Gastric Carcinoma by Polymerase Chain Reaction
- Isolation of Differentiated Squamous and Undifferentiated Spindle Carcinoma Cell Lines with Differing Metastatic Potential from a 4-Nitroquinoline N-Oxide-induced Tongue Carcinoma in a F344 Rat
- Adenovirus-Mediated Transduction of Escherichia coli Uracil Phosphoribosyltransferase Gene Increases the Sensitivity of Esophageal Cancer Cells to 5-Fluorouracil
- Construction of a Practical SCAR Marker Linked to Clubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes
- Solitary bone metastasis as the first clinical manifestation in a patient with small bowel adenocarcinoma
- Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes
- Cloning of the SAT1 Gene Concerned with Salt Tolerance of the Yeast Zygosaccharomyces rouxii
- Cloning and analysis of genes coding for chitinolytic enzymes from the novel chitinolytic bacterium Chitiniphilus shinanonensis
- Phase II Trial of 5-Fluorouracil and Low-Dose Cisplatin in Patients with Squamous Cell Carcinoma of the Esophagus
- Alternative Splicing of the FHIT Gene in Colorectal Cancers
- Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes
- Characterization of a novel lymph node metastasis model from human colonic cancer and its preclinical use for comparison of anti-metastatic efficacy between oral S-1 and UFT/LV
- COUMARIN UP-TAKE SYSTEM IN TOBACCO CULTURED CELL
- INDUCTION AND GENETIC ANALYSIS OF PHENYLALANINE AMMONIA-LYASE IN TOBACCO COULTURED CELL
- Metabolism of administered (2RS)-naringenin in flavonoidproducingcultured cells of Sophora flavescens
- Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae
- Purification and Some Properties of 2, 4-D-Activated Scooletin Glucosyltransferase from Tobacco Cells
- COLLECTION AND ANALYSIS OF ARABIDOPSIS COLOR MUTANTS
- Analysis of transposon Tag1 related elements
- Characterization of the Arabidopsis thaliana Mutant pcb2 which Accumulates Divinyl Chlorophylls
- HETEROLOGOUS EXPRESSION AND PROPERTIES OF GLUCOSYLTRANSFERASE NTGT1 FROM TOBACCO
- THE EFFECT OF METHYL JASMONATE ON 2,4-D STIMULATED SCOPOLETIN UPTAKE BY TOBACCO CELLS
- 325 Regulation of Phenylalanine Ammonia-lyase in Tobacco Cell Culture
- Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae
- Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis
- Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
- Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Distribution of CRa in clubroot resistance (CR) cultivars of Chinese cabbage