Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
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概要
- 論文の詳細を見る
A gene (cob) conferring red fluorescence on Escherichia coli cells was isolated from an environmental DNA library constructed from soil bacteria. The nucleotide sequence showed a single open reading frame (ORF) encoding a polypeptide of 257 amino acid residues responsible for the red fluorescence. The deduced amino acid sequence of the ORF showed significant similarity (less than 75% identity) to uroporphyrinogen III methyltransferases from various bacteria. Cell extracts from the E. coli transformant had a spectrum pattern of fluorescence corresponding to trimethylpyrrocorphin or sirohydrochlorin that was absent in the control cells harboring the vector alone. Expression of the cob gene in the fungus Fusarium oxysporum conferred red fluorescence on the host cells, indicating that it is a promising transcriptional reporter in fungi as well as bacteria.
- 社団法人 日本農芸化学会の論文
著者
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SHIMOSAKA MAKOTO
Department of Applied Biology, Shinshu University
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Taguchi G
信州大・繊維・応生系
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Sato Kazuaki
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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LAN Xiqian
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Sato Kazuaki
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Lan Xiqian
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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TAGUCHI Goro
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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ZHOU Zeyang
Sericultural Key Laboratory of the Agricultural Ministry, Southwest University
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Zhou Zeyang
Sericultural Key Laboratory Of The Agricultural Ministry Southwest University
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Sato Kazuaki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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