Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus

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概要

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• A new exo-type cellulase, named exo-cellulase II(Ex-2), was purified from the crude enzyme preparation of Irpex lacteus. Ex-2 was very similar to the previously characterized exo-cellulase I(Ex-1)with respect to enzymatic features such as optimal pH, temperature, heat stability, and catalytic activity. However, Ex-2 exhibited greater pH stability than Ex-1. The molecular mass and carbohydrate content to Ex-2(56, 000, 4.0%)were different from those of Ex-1(53, 000, 2.0%). A cellulase gene(named cel2)encoding both Ex-2 and Ex-1 was isolated from an I.lacteus genomic library. The cel2 gene was found to consist of 1569 bp with an open reading frame encoding 523 amino acids, interrupted by two introns. The deduced amino acid sequences revealed that cel2 ORF has a modular structure consisting of a catalytic domain and a fungal-type cellulose-binding domain(CBD)separated by a serine-rich linker region. The catalytic domain was homologous to those of fungal cellubiohydrolases belonging to family 7 of the glycosyl hydrolases. Northern blot analysis showed that expression of the cel2 gene was induced by various cellulosic substrates and repressed by glucose, fructose, and lactose.

• 社団法人日本生物工学会の論文
• 1999-04-25

著者

• Hamada N

  Laboratory Of Applied Microbiology Graduate School Of Marine Science And Technology Tokyo University

• OKAZAKI MITSUO

  Department of Applied Biology, Shinshu University

• SHIMOSAKA MAKOTO

  Department of Applied Biology, Shinshu University

• Shiroishi Masahiro

  Department Of Physics Graduate School Science University Of Tokyo

• KANDA Takahisa

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• AMANO Yoshihiko

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Kanda Takahisa

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Amano Yoshihiko

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Shimosaka M

  信州大・繊維・応生系

• Shimosaka Makoto

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• KODAIRA Ritsuko

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• HAMADA NAOKO

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• ISHIKAWA KAZUYA

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• FUSE NAOKI

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• Ishikawa Kazuya

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Okazaki M

  Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• 城石 正弘

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Fuse Naoki

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Arikawa Y

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Kodaira Ritsuko

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kuroyanagi Tomoko

  Faculty Of Textile Science And Technology Shinshu University

• Ishikawa Kazuya

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

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